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A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria


Citation

Abd. Shukor, Mohd. Yunus and Shamsuddin, Burhanuddin and Mohamad, Othman and Ithnin, Khalid and Shamaan, Nor Aripin and Syed, Mohd. Arif (2008) A method to study the effects of chemical and biological reduction of molybdate to molybdenum blue in bacteria. Pakistan Journal of Biological Sciences, 11 (4). pp. 672-675. ISSN 1028-8880; ESSN: 1812-5735

Abstract

In this research, we modify a previously developed assay for the quantification molybdenum blue to determine whether inhibitors to molybdate reduction in bacteria inhibits cellular reduction or inhibit the chemical formation of one of the intermediate of molybdenum blue; phosphomolybdate. We manage to prove that inhibition of molybdate reduction by phosphate and arsenate is at the level of phosphomolybdate and not cellular. We also prove that mercury is a physiological inhibitor to molybdate reduction. We suggest the use of this method to assess the effect of inhibitors and activators to molybdate reduction in bacteria.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.3923/pjbs.2008.672.675
Publisher: Asian Network for Scientific Information
Keywords: Molybdate reduction; E. cloacae strain 48; Molybdenum blue; Inhibitors
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 29 May 2015 05:32
Last Modified: 02 Dec 2015 03:29
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3923/pjbs.2008.672.675
URI: http://psasir.upm.edu.my/id/eprint/12722
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