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Effects of controlled water activity on microbial community succession and flavor formation in low-salt chili mash fermentation


Citation

Dai, Linli and Wang, Xin and Ahmad, Nurul Hawa and Mah, Jae Hyung and Qin, Wen and Wei, Xinyao and Liu, Shuxiang (2026) Effects of controlled water activity on microbial community succession and flavor formation in low-salt chili mash fermentation. Foods, 15 (2). art. no. 360. pp. 1-17. ISSN 2304-8158

Abstract

Although fermented seasonings play a pivotal role in improving food quality, the high sodium content of many traditional products poses considerable public health concerns, including hypertension and cardiovascular disease. This study established a low-salt fermentation strategy for Mumashan chili by regulating water activity (aw) under NaCl concentrations ranging from 4 to 12% (w/w). The aw-regulated system effectively maintained aw within ± 0.03 at both 25 and 40 °C, thereby sustaining stable microbial activity despite the reduced salt concentration. Compared with the control group 15% NaCl, the 4% NaCl treatments exhibited significantly higher total acidity (130–200 g/kg vs. 24–58 g/kg) and a faster consumption rate of reducing sugars, with MH12 achieving an 80% rate of reducing sugars by day 21. Sensory evaluation revealed a higher overall quality score for the low-salt chili mash (MH12, 7.7/10), which was associated with a balanced aroma profile and enhanced color stability (ΔE < 5). However, the elevated relative abundance of opportunistic pathogens (Klebsiella app., ~10%) highlights the necessity of strict raw material hygiene. Overall, these results validate the feasibility of aw regulation for low-salt fermentation, elucidate the associations between microbial communities and flavor development, and provide a basis for future industrial applications.


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Additional Metadata

Item Type: Article
Subject: Food Science
Subject: Microbiology
Divisions: Faculty of Food Science and Technology
DOI Number: https://doi.org/10.3390/foods15020360
Publisher: Multidisciplinary Digital Publishing Institute
Keywords: Low-salt chili mash; Microbial diversity; Volatile flavor substance; Water activity
Depositing User: Mr. Mohamad Syahrul Nizam Md Ishak
Date Deposited: 16 Mar 2026 00:45
Last Modified: 16 Mar 2026 00:45
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/foods15020360
URI: http://psasir.upm.edu.my/id/eprint/123595
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