Citation
Subramaniam, Chandrasekaran
(1993)
Pasteurella Multocida Type B In Malaysia: Characterisation, Immune Response And Protection In Vaccinated Buffaloes.
Masters thesis, Universiti Pertanian Malaysia.
Abstract
A study on the characteristics of the Malaysian field and
vaccine strains of Pasteurella multocida type B was conducted .
In addition , the immunogenic potential of haemorrhagic
septicaemia ( HS ) vaccines was evaluated in buffaloes .
A total of 48 P. multocida isolates including the five HS
vaccine strains were included in the study . Initial studies
showed significant cross-protection in mice amongst the five
vaccine strains . The 48 isolates , when subjected to
biochemical tests had very little or no variability amongst
them. Type-specific hyper immune sera were prepared in rabbits
and buffaloes . The sera were then absorbed using antigens
prepared from the 48 isolates . A series of serological tests
were conducted using the pre- and post-absorbed sera and the
antigen preparations . The protein profile of the five vaccine
and five randomly selected field strains of this organism
revealed that there appeared to be no variation amongst the
field and vaccine strains .
The antibody responses of buffaloes immunised with three
conventional HS vaccines viz . , broth bacterin ( BB ) ,
alum-precipitated vaccine ( APV ) and oil adjuvant vaccine ( OAV )
and one experimental double emulsion vaccine ( DEV ) were
determined by an enzyme-linked immunosorbent assay ( ELISA ) .
Antibody levels were significantly higher in buffaloes
immunised with the adjuvanted vaccines ( APV , OAV , DEV ) .
Buffaloes immunised with BB alone were protected at 6 weeks but
not at 12 weeks post-immunisation . Buffaloes immunised with
APV were protected at 24 weeks but only partially at 52 weeks
post-immunisation . On the other hand , buffaloes immunised with
OAV or DEV were fully protected at 52 weeks but only partially
at 76 weeks post-immunisation .There was also a positive
relationship between the ELISA titres and protection in
buffaloes .
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