Comparative genome analysis and genome mining of Lacticplantibacillus plantarum PA21: insights into its antimicrobial potential

myThe dramatic increase of antimicrobial resistance in the healthcare realm has become inexorably linked to theabuse of antibiotics over the years. With resistance continuing to evolve, the need for alternative antimicrobialagents is of utmost priority. In the present work, whole genome sequencing was done on Lactiplantibacillusplantarum PA21 using Oxford Nanopore sequencing technology. Comparative genomics through averagenucleotide identity (ANI) and phylogenomic analysis was performed to investigate genomic linkage, speciesboundaries and to gain insights into the evolutionary relationship of L. plantarum PA21. Additionally, the genomeof L. plantarum PA21 was screened for potential probiotic genes through the NCBI database. The BAGEL4 andantiSMASH databases were used to mine putative bacteriocin clusters and genes related to the biosynthesis ofantimicrobial proteins. Then, agar well diffusion method was performed to evaluate the antimicrobial activity ofL. plantarum PA21 against multidrug resistant pathogens. The genome sequence of L. plantarum PA21 consistingof three contigs was assembled to a size of 3,218,706 bp. Phylogenomic analysis and average nucleotide identity(ANI) revealed L. plantarum PA21 is closely related to genomes isolated from diverse niches such as dairyproducts, food, and animals. Genome mining through the BAGEL4 and antiSMASH databases revealed fourbacteriocins in a single cluster and four regions of biosynthetic gene clusters responsible for the production ofbioactive compounds. It was demonstrated through agar well diffusion assay that the L. plantarum PA21 cell-freesupernatant exhibited antimicrobial activity against all nine methicillin-resistant Staphylococcus aureus (MRSA)and three out of 13 Klebsiella pneumoniae multidrug resistant clinical isolates. Results from this study demonstratethat L. plantarum PA21 CFS is a prolific and potential source of antimicrobial against multi-drug resistantpathogens.

Text 121407.pdf - Published Version Restricted to Repository staff only Download (796kB)

Actions (login required)

View Item