Characterization of a virulent phage, P12L (genus Drulisvirus), targeting Klebsiella pneumoniae capsule type K2

Citation

Rollon, Wendy Dayang and Dean, Tay Darren and Mohd Idris, Sharifah Ishaziah and Mazlan, Nurzafirah and Jati, Afif Pranaya and Thung, Tze Young (2025) Characterization of a virulent phage, P12L (genus Drulisvirus), targeting Klebsiella pneumoniae capsule type K2. Archives of Virology, 170 (6). art. no. 128. pp. 1-5. ISSN 0304-8608; eISSN: 1432-8798

Abstract

A virulent bacteriophage, P12L, infecting hypermucoviscous Klebsiella pneumoniae of capsule-type K2 was characterized. The phage was found to have podovirus-like morphology, with an icosahedral head and a short tail. It exhibited efficient adsorption with a burst size of 183 PFU/cell. The viral genome is a linear dsDNA molecule that is 42,343 bp in length and contains 62 putative open reading frames (ORFs). It lacks genes associated with drug resistance or virulence factors and encodes two predicted domains associated with depolymerase activity. Because depolymerase can degrade polysaccharide capsules and promote efficient phage-host interactions, phage P12L shows potential as a biocontrol agent.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Food Science and Technology
DOI Number:	https://doi.org/10.1007/s00705-025-06320-7
Publisher:	Springer
Keywords:	Autoscriptoviridae; Depolymerase; Drulisvirus; Klebsiella pneumoniae; Phage
Depositing User:	Ms. Nuraida Ibrahim
Date Deposited:	30 Oct 2025 03:56
Last Modified:	30 Oct 2025 03:56
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s00705-025-06320-7
URI:	http://psasir.upm.edu.my/id/eprint/121279
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