Citation
Bein, Bernhard and Chrysostomakis, Ioannis and Arantes, Larissa S. and Brown, Tom and Gerheim, Charlotte and Schell, Tilman and Schneider, Clément and Leushkin, Evgeny and Chen, Zeyuan and Sigwart, Julia and Gonzalez, Vanessa and Wong, Nur Leena W.S. and Santos, Fabricio R. and Blom, Mozes P.K. and Mayer, Frieder and Mazzoni, Camila J. and Böhne, Astrid and Winkler, Sylke and Greve, Carola and Hiller, Michael
(2025)
Long-read sequencing and genome assembly of natural history collection samples and challenging specimens.
Genome Biology, 26 (1).
art. no. 25.
pp. 1-25.
ISSN 1474-7596; eISSN: 1474-760X
Abstract
Museum collections harbor millions of samples, largely unutilized for long-read sequencing. Here, we use ethanol-preserved samples containing kilobase-sized DNA to show that amplification-free protocols can yield contiguous genome assemblies. Additionally, using a modified amplification-based protocol, employing an alternative polymerase to overcome PCR bias, we assemble the 3.1 Gb maned sloth genome, surpassing the previous 500 Mb protocol size limit. Our protocol also improves assemblies of other difficult-to-sequence molluscs and arthropods, including millimeter-sized organisms. By highlighting collections as valuable sample resources and facilitating genome assembly of tiny and challenging organisms, our study advances efforts to obtain reference genomes of all eukaryotes.
Download File
Additional Metadata
Actions (login required)
 |
View Item |
![[img]](http://psasir.upm.edu.my/style/images/fileicons/text.png)