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Transient prenatal ruxolitinib treatment promotes neurogenesis and suppresses astrogliogenesis during embryonic mouse brain development


Citation

Abdullah, Amirah Nabilah and Fasihah Zamri, Auni and Alias, Khairunnisa and Azhar, Nur Munawwarah and Ling, King Hwa and Cheah, Pike See and Zainal Abidin, Shahidee (2025) Transient prenatal ruxolitinib treatment promotes neurogenesis and suppresses astrogliogenesis during embryonic mouse brain development. Neuroscience Research Notes, 8 (1). art. no. 352. pp. 1-7. ISSN 2576-828X

Abstract

Ruxolitinib is a Janus kinase (JAK) inhibitor that inhibits the JAK/STAT signalling pathway by targeting JAK1 and JAK2, which are crucial for regulating astrogliogenesis. This study assessed the effect of ruxolitinib (5 and 30 mg/kg/day) on developing mouse brains by administering it to pregnant mice from E7.5 to E20.5. No adverse effects were observed in the treated mice. The brains of P1.5 pups were collected, and RNA was extracted to assess markers of neurogenesis and astrogliogenesis through RT-qPCR. The results revealed a significant decrease in Gfap expression (p<0.0001) in both ruxolitinib-treated groups compared to the control, indicating a suppression of astrogliogenesis. Additionally, S100β expression (p<0.05) was significantly reduced in the 30 mg/kg ruxolitinib-treated group. In contrast, the expression of neuronal markers vGLuT1 (p<0.01) and vGLuT2 (p<0.01) increased significantly in the 30 mg/kg treated group, suggesting enhanced neuronal differentiation. Furthermore, 5 and 30 mg/kg ruxolitinib-treated groups showed a significant increase in GAT1 expression (p<0.01) compared to the control group. A marked decrease in Nestin expression was also observed in the 5 mg/kg (p<0.001) and 30 mg/kg (p<0.0001) treated groups. These findings demonstrate that transplacental administration of ruxolitinib modulates key markers involved in neuronal differentiation and gliogenesis in the developing mouse brain, suggesting its potential use in correcting imbalances in early brain development.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Malaysian Research Institute on Ageing
DOI Number: https://doi.org/10.31117/neuroscirn.v8i1.352
Publisher: Neurotak Publishing
Keywords: Astrogliogenesis; Down syndrome; JAK-STAT pathway; Neurogenesis; Ruxolitinib
Depositing User: Ms. Zaimah Saiful Yazan
Date Deposited: 23 Jul 2025 02:08
Last Modified: 23 Jul 2025 02:08
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.31117/neuroscirn.v8i1.352
URI: http://psasir.upm.edu.my/id/eprint/118733
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