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Broccoli stem extract enhances browning inhibition in fresh-cut peach fruit by inhibiting polyphenol oxidase activity and improving antioxidant capacity


Citation

Zhang, Fengru and Jiang, Shu and Jia, Shuang and Gui, Boya and Wei, Yingying and Chen, Yi and Ye, Jianfen and Xu, Feng and Ding, Phebe and Shao, Xingfeng (2025) Broccoli stem extract enhances browning inhibition in fresh-cut peach fruit by inhibiting polyphenol oxidase activity and improving antioxidant capacity. Postharvest Biology and Technology, 222. art. no. 113399. pp. 1-13. ISSN 0925-5214; eISSN: 0925-5214

Abstract

This study focused on the anti-browning effect of broccoli stem extract (BE) on fresh-cut peaches. Compared to L-cysteine (L-Cys), BE showed the higher inhibition of the surface browning in fresh-cut peach fruit. This was accompanied with a more decreased polyphenol oxidase (PPO) activity and down-regulation of PpPPO expression. BE showed a non-competitive inhibition for peach PPO, and the highest inhibition rate (85.23 ± 3.86 %) was found when PPO catalyzed the oxidation of catechol. Furthermore, sulfur-containing compounds, including methylthiouracil (MTU), S-Methyl methanethiosulfonate (MMTS) and thiophene, 2-propyl- were identified in BE and shown to inhibit PPO activity. The molecular docking also indicated that these three compounds spontaneously bound PPO via hydrogen bonding, π-π conjugation and hydrophobic interactions, resulting in the PPO inhibition and its associated browning. BE also increased the total phenol and flavonoids content as well as antioxidant capacities in fresh-cut fruit, suggested by increased activities of catalase (CAT), ascorbate peroxidase (APX) and 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging rate. Therefore, BE could be a natural anti-browning agent that can be used for the inhibition of PPO-initiated discoloration in peach fruit.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
DOI Number: https://doi.org/10.1016/j.postharvbio.2025.113399
Publisher: Elsevier B.V.
Keywords: Anti-browning; Peach; Plant extract; Polyphenol oxidase (PPO); Sulfur-containing compounds
Depositing User: Mohamad Jefri Mohamed Fauzi
Date Deposited: 21 Jul 2025 06:50
Last Modified: 21 Jul 2025 06:50
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.postharvbio.2025.113399
URI: http://psasir.upm.edu.my/id/eprint/118654
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