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Pineapple peel bromelain extract improves poultry in vitro protein digestibility


Citation

Akit, Henny and Loh, Teck Chwen and Ab Aziz, Muhamad Faris and Foo, Hooi Ling and Zailani, N. S. (2024) Pineapple peel bromelain extract improves poultry in vitro protein digestibility. Malaysian Journal of Animal Science, 27 (2). pp. 53-64. ISSN 1394-3227; eISSN: 2550-2123

Abstract

This study aimed to assess the proteolytic activity of pineapple peel crude bromelain extracts using different extractants and its impact on in vitro protein digestibility, simulating poultry digestion. This study compared the proteolytic activity of bromelain extracted using deionized water alone and in combination with 0.02 M dithiothreitol (DTT), 0.02% sodium azide, and 0.02 M ethylenediaminetetraacetic acid (EDTA). Subsequently, the effects of bromelain extracts on protein solubilization and digestibility using a poultry gut simulation model were evaluated. The findings indicated that bromelain extractions using deionized water (82.22 U/mg) and a combination of deionized water + sodium azide + EDTA (87.84 U/mg) exhibited the highest proteolytic activities compared to other extractants (P<0.05). Furthermore, pineapple peel bromelain extracts resulted in up to 24% increase in protein solubility and up to 6% increase in protein digestibility (P<0.05, respectively). Notably, bromelain extracted using deionized water (60.69 U/mg) proved to be the most effective in digesting protein in vitro.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
Publisher: Malaysian Society of Animal Production
Keywords: Protease; Pineapple waste; Proteolytic activity; Chicken
Depositing User: Ms. Che Wa Zakaria
Date Deposited: 01 Jul 2025 06:54
Last Modified: 01 Jul 2025 06:54
URI: http://psasir.upm.edu.my/id/eprint/118225
Statistic Details: View Download Statistic

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