Immunohistochemical detection of African swine fever and classical swine fever virus antigen from formalin-fixed paraffin-embedded swine tissues

Citation

Uda Zahli, Nurul Izzati and Che' Amat, Azlan and Fong, Michelle Wai Cheng and Ooi, Peck Toung and Phan, Y.W. (2024) Immunohistochemical detection of African swine fever and classical swine fever virus antigen from formalin-fixed paraffin-embedded swine tissues. Jurnal Veterinar Malaysia, 36 (1). pp. 25-29. ISSN 9128-2506

Abstract

African swine fever was first reported in February 2021 and shares similar pathological signs with classical swine fever, which is endemic in Malaysia. This study aimed to detect the viral antigen in formalin-fixed paraffin-embedded (FFPE) tissue samples using immunohistochemistry and to characterise the histopathological lesions. The FFPE tissues of spleen, tonsil and lymph nodes of 17 domestic pigs and three wild boars were collected from 2020 to 2022 from the histopathology laboratory archive. Immunohistochemistry revealed ASFV antigen-positive cells in 1/20 (5%) samples and CSFV antigen-positive cells in 4/20 (20%) samples. All CSF-positive samples show lymphoid depletion in the tonsil, lymph node and spleen tissues and 3/4 (75%) show haemorrhages in lymph node and spleen tissues. This study illustrates the need to strengthen biosecurity to prevent transmission of ASF and CSF into the farm.

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Official URL or Download Paper: https://www.mavma.org.my/jvm/2024-volume-36-issue-...

Additional Metadata

Item Type:	Article
Divisions:	Faculty of Veterinary Medicine
Publisher:	Malaysian Veterinary Medical Association
Keywords:	African swine fever; Classical swine fever; Immunohistochemistry; Porcine
Depositing User:	Ms. Zaimah Saiful Yazan
Date Deposited:	10 Jul 2025 03:19
Last Modified:	10 Jul 2025 03:19
URI:	http://psasir.upm.edu.my/id/eprint/118153
Statistic Details:	View Download Statistic

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