Citation
Abstract
Vitex trifolia var. purpurea leaves (Lemuni leaves) is considered as a traditional medicine due to their antioxidant property. The antioxidant properties of Lemuni leaves depends on the concentration of ethanol-water solvent. Therefore, this current study intended to identify the antioxidant activity of Lemuni leaves (Vitex trifolia var. purpurea) in different concentrations of ethanol-water solvent extraction. Four ethanol-water concentrations were prepared (water extract (0% ethanol), 25% ethanol extract, 50% ethanol extract, and 75% ethanol extract). The antioxidant properties of Lemuni leaves were evaluated by FolinCiocalteu assay, aluminium chloride calorimetric method while 2,2’-diphenyl-1picrylhydrazyl (DPPH) assay and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay were used to measure the antioxidant activities of the extracts. The experimental results showed that 75% ethanol extract had the highest (p<0.05) total phenolic content (94.69 ± 4.75mg GAE/g DW) and total flavonoid content (30.76 ± 2.85mg QE/g DW) significantly. The 75% ethanol extract also had the significantly lowest (p<0.05) IC50 value for DPPH (3.56 ± 0.11 mg/mL) and ABTS (2.01 ± 0.05 mg/mL) assays. Strong correlations were discovered between the total flavonoid content, total phenolic content with antioxidant properties of Lemuni leaves at different ethanol-water ratio by DPPH (r = -0.816; r = -0.824) and ABTS assays (r = -0.929; r = -0.920). The significantly elevated antioxidant properties of the 75% ethanol extract suggest promising applications in health supplements, pharmaceuticals, skincare products, biomedical research, and commercial ventures.
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Official URL or Download Paper: https://talenta.usu.ac.id/InJAR/article/view/13357
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Additional Metadata
Item Type: | Article |
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Divisions: | Faculty of Medicine and Health Science Halal Products Research Institute |
DOI Number: | https://doi.org/10.32734/injar.v7i2.13357 |
Publisher: | Talenta Publisher |
Keywords: | Antioxidant activity; Ethanol-water solvent; Lemuni; Total phenolic content; Total flavonoid content |
Depositing User: | Ms. Zaimah Saiful Yazan |
Date Deposited: | 30 May 2025 02:21 |
Last Modified: | 30 May 2025 02:21 |
Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.32734/injar.v7i2.13357 |
URI: | http://psasir.upm.edu.my/id/eprint/117582 |
Statistic Details: | View Download Statistic |
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