UPM Institutional Repository

Proteomic and morphological analysis of bone and articular cartilage changes in osteoarthritic rabbits supplemented with Edible Bird’s Nest (EBN)


Citation

Sulaiman, Sharifah Zakiah Syed and Umran, Nor Shahira Solehah and Mohamed, Suhaila and Abu, Jalila and Ng, Angela Min Hwei and Lau, Benjamin Yii Chung and Lai, Kok Song and Ilias, Nazhan and Lau, Seng Fong and Ajat, Mokrish (2024) Proteomic and morphological analysis of bone and articular cartilage changes in osteoarthritic rabbits supplemented with Edible Bird’s Nest (EBN). Malaysian Journal of Fundamental and Applied Sciences, 20 (6). pp. 1274-1287. ISSN 2289-599X; eISSN: 2289-5981

Abstract

This study is aimed at assessing the effects of Edible Bird’s Nest (EBN) on subchondral bone, articular cartilage and the expression of proteins in synovial fluid by micro-CT evaluation and histological analysis. 54 New Zealand white rabbits were induced by intra-articular injection of monosodium iodoacetate (8 mg) and divided into four groups: negative control (n=9): non-treated osteoarthritis; positive control (n=15): OA + diclofenac sodium 2 mg/kg daily orally; low dosage (n=15): OA + 75 mg/kg hydrolyzed EBN; and high dosage (n=15): OA + 150 mg/kg hydrolyzed EBN. The joints were harvested and subjected to micro-CT analysis and histological evaluation, and the synovial fluid was subjected to LCMS/MS analysis. Micro-CT analysis showed an increase in bone volume and a decrease in total porosity in the treatment group that showed bone integrity improvement. Histopathological results revealed comparable changes between the positive control group and the EBN treatment group. There was upregulation of proteins involved in the resolution of inflammation and downregulation of proteins associated with the bone resorption process. Morphology evaluation showed that EBN supplementation has a bone-improving effect by inhibiting osteoclastic activity. Protein expression showed chondroprotection and bone improvement through the action of several proteins via various signaling pathways. The morphological and molecular findings suggest the potential use of EBN as beneficial alternative for osteoarthritis treatment by improving bone quality and modulating inflammatory responses.


Download File

[img] Text
117537.pdf - Published Version

Download (1MB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Institute of Bioscience
DOI Number: https://doi.org/10.11113/mjfas.v20n6.3634
Publisher: Penerbit UTM Press
Keywords: Articular cartilage; Edible bird’s nest (EBN); Micro-computed tomography (micro-CT); Osteoarthritis (OA); Subchondral bone
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 29 May 2025 03:05
Last Modified: 29 May 2025 03:05
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.11113/mjfas.v20n6.3634
URI: http://psasir.upm.edu.my/id/eprint/117537
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item