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Cellular and humoral responses in the respiratory tract of goats following intranasal stimulation using formalin-killed Pasteurella haemolytica A2


Citation

Zamri-Saad, M. and Effendy, A.W.M. and Israf, D.A. and Azmi, M.L. (1999) Cellular and humoral responses in the respiratory tract of goats following intranasal stimulation using formalin-killed Pasteurella haemolytica A2. Veterinary Microbiology, 65 (3). pp. 233-240. ISSN 0378-1135

Abstract

A study to determine the immunoglobulin and cellular responses in the respiratory tract of goats following intranasal exposures to formalin-killed Pasteurella haemolytica A2 was carried out. Forty-two goats were divided into two groups. Goats in Group 1 were subjected to double intranasal exposures to formalin-killed P. haemolytica A2 while goats in Group 2 were the unexposed control. Prior to and at weekly intervals post-exposure, three goats from each group were killed, serum samples were collected while the lungs were flushed with 50ml normal saline before the right apical lobes were fixed in 10% buffered formalin. Both serum and lung lavage fluid were subjected to enzyme-linked immunosorbent assay (ELISA) to determine the levels of IgA, IgM and IgG while the formalin-fixed tissues were examined histologically. IgA levels in the lung lavage fluid increased rapidly to reach a significantly (p<0.05) high level as early as Week 2 post-exposure and remained significantly (p<0.05) high throughout the study period. The IgM levels increased at an intermediate rate to reach a significantly (p<0.05) high level at Week 3 post-exposure before they decreased to an insignificant (p>0.05) level the following week and the weeks thereafter. IgG levels increased gradually and only reached a significantly (p<0.01) high level at Weeks 5 and 6 of the study. The size of the bronchus-associated lymphoid tissue (BALT) and the number of lymphocytes in BALT increased significantly from Week 2 and remained high thereafter. However, differences in the numbers of BALT were insignificant (p>0.05) initially before becoming significantly (p<0.05) high at Weeks 5 and 6. The BALT responses were parallel to those of immunoglobulins in the lung lavage fluid.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.1016/S0378-1135(98)00298-3
Publisher: Elsevier Science B.V.
Keywords: Goat; Immune response-goat; Intranasal stimulation; Pasteurella haemolytica
Depositing User: Ms. Zaimah Saiful Yazan
Date Deposited: 04 Jul 2025 08:31
Last Modified: 04 Jul 2025 08:31
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/S0378-1135(98)00298-3
URI: http://psasir.upm.edu.my/id/eprint/116752
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