UPM Institutional Repository

Cloning and expression of a novel lipase gene from Bacillus sphaericus 205y


Citation

Rahman, R.N.Z.A. and Chin, J.H. and Salleh, A.B. and Basri, M. (2003) Cloning and expression of a novel lipase gene from Bacillus sphaericus 205y. Molecular Genetics and Genomics, 269 (2). pp. 252-260. ISSN 1617-4615

Abstract

A Bacillus sphaericus strain (205y) that produces an organic solvent-tolerant lipase was isolated in Port Dickson, Malaysia. The gene for the lipase was recovered from a genomic library and sequenced. Phylogenetic analysis was performed based on an alignment of thirteen microbial lipase sequences obtained from the NCBI database. The analysis suggested that the B. sphaericus lipase gene is a novel gene, as it is distinct from other lipase genes in Families 1.4 and 1.5 reported so far. Expression in Escherichia coli under the control of the lacZ promoter resulted in an eight-fold increase in enzyme activity after a 3-h induction with 1 mM IPTG. The crude enzyme thus obtained showed a slight (10%) enhancement in activity after a 30-min incubation in 25% (v/v) n -hexane at 37°C, and retained 90% of its activity after a similar period in 25% (v/v) p-xylene.


Download File

[img] Text
116742.pdf - Published Version
Restricted to Repository staff only

Download (474kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Environmental Studies
DOI Number: https://doi.org/10.1007/s00438-003-0831-5
Publisher: Springer Verlag
Keywords: Bacillus sphaericus; Lipase gene; Organic solvent-tolerant lipase; Phylogenetic tree
Depositing User: Ms. Zaimah Saiful Yazan
Date Deposited: 07 Jul 2025 06:29
Last Modified: 07 Jul 2025 06:29
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s00438-003-0831-5
URI: http://psasir.upm.edu.my/id/eprint/116742
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item