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Effect of pH, temperature and agitation on thermophilic biohydrogen production using immobilized cells on carbon composites (GAC-NiFe3O4)


Citation

Mohd Jamaludin, Nina Farhana and Engliman, Nurul Sakinah and Abdul Manaf, Shareena Fairuz and Idrus, Syazwani and Abdullah, Luqman Chuah and Jamali, Nur Syakina (2024) Effect of pH, temperature and agitation on thermophilic biohydrogen production using immobilized cells on carbon composites (GAC-NiFe3O4). Chemical Engineering Journal, 485. art. no. 149980. pp. 1-11. ISSN 1385-8947; eISSN: 1385-8947

Abstract

Carbon composites-immobilized cell was utilized to enhance biohydrogen production. The initial pH, temperature and agitation effect was studied using the one-factor-at-a-time (OFAT) method. The optimal initial pH obtained was at pH 6.0 with hydrogen yield (HY) of 2.66 ± 0.09 mol H2/mol sugar, correlating with Gompertz constant of Hm = 974.99 mL, Rm = 17.90 mL/h and λ = 1.70 h. At 60 °C, the highest HY was obtained at 2.75 ± 0.11 mol H2/mol sugar. These corresponded to the Gompertz constant of Hm = 934.58 mL, Rm = 18.78 mL/h and λ = 1.89 h. The optimal agitation obtained was at 120 rpm, attaining the highest HY of 3.44 ± 0.54 mol H2/mol sugar, corresponding to the Gompertz constant of Hm = 1054.86 mL, Rm = 24.37 mL/h and λ = 3.90 h. The rRNA sequencing result revealed that the predominant species in the study was Thermoanaerobacterium. This study provides critical insight into process control conditions on biohydrogen production.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
Institut Nanosains dan Nanoteknologi
DOI Number: https://doi.org/10.1016/j.cej.2024.149980
Publisher: Elsevier
Keywords: Dark fermentation; Granular activated carbon nanocomposites; Immobilized cell; Process parameter; Thermophilic biohydrogen production
Depositing User: Ms. Che Wa Zakaria
Date Deposited: 07 Jul 2025 08:26
Last Modified: 07 Jul 2025 08:26
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.cej.2024.149980
URI: http://psasir.upm.edu.my/id/eprint/116600
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