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Surface-Enhanced Raman spectroscopy (SERS) substrates based on photonic crystal embedded bi-metallic nanoparticles for leptospiral DNA detection


Citation

Abdul Razak, Anis Athirah and Shatar, Liyana and Ramli, Aima and Kassim, Syara and Mohd Ghazali, Mohd Sabri and Chee, Hui Yee and Zakaria, Rozalina and Mahdi, Mohd Adzir and Suhailin, Fariza Hanim (2024) Surface-Enhanced Raman spectroscopy (SERS) substrates based on photonic crystal embedded bi-metallic nanoparticles for leptospiral DNA detection. Applied Spectroscopy. ISSN 0003-7028; eISSN: 1943-3530

Abstract

Leptospirosis is an acute bacterial febrile disease affecting humans and animals in many tropical and subtropical countries. This work presents an optimization of surface-enhanced Raman spectroscopy (SERS) substrates to probe vibrational spectroscopic detail from Leptospira deoxyribonucleic acid (DNA). The pathogenic gene of LipL32 was used as a biomarker. The SERS substrates were based on a photonic crystal (PC) structure embedded with bi-metallic gold and silver nanoparticles (PC@AuAg NPs). The localized plasmonic resonance of AuAg NPs was coupled to the Raman modes of the target through SERS interaction. Prior to detection, the AuAg NPs were functionalized with chemical linkers to facilitate specific conjugation between metallic surfaces and DNA biomolecules. The immobilization and hybridization of probe DNA to their complementary target DNA (cDNA) created duplex formation for detection. The configuration was also tested with non-complementary DNA to verify detection specificity. Prominent SERS peaks were recorded, and the characteristic intensity decreased after cDNA hybridization due to less base interaction after complementary pairing. Distinct SERS behavior from the negative control test was also observed in non-complementary interaction. The configuration is highly attractive and can be potentially extended for sensitive and label-free detection of leptospiral DNA, paving the way for alternative diagnosis of leptospirosis.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1177/00037028241303780
Publisher: SAGE Publications
Keywords: Leptospirosis; Nanoparticles; Photonic crystal; SERS; Surface-enhanced Raman spectroscopy
Depositing User: Ms. Che Wa Zakaria
Date Deposited: 07 Jul 2025 09:11
Last Modified: 07 Jul 2025 09:11
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1177/00037028241303780
URI: http://psasir.upm.edu.my/id/eprint/116476
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