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Characterisation and molecular cloning of an erythromycin resistance plasmid of Lactococcus lactis isolated from chicken cecum


Citation

A.R., Raha and Ross, E. and Yusoff, K. and Manap, M.Y. and Ideris, A. (2002) Characterisation and molecular cloning of an erythromycin resistance plasmid of Lactococcus lactis isolated from chicken cecum. Journal of Biochemistry, Molecular Biology and Biophysics, 6 (1). pp. 7-11. ISSN 1025-8140; eISSN: 1025-8140

Abstract

An erythromycin resistance plasmid, pAJ01 was isolated from Loctococcus lactis isolate C5 that was isolated from a healthy two-week-old chicken cecum. A 4 kb plasmid was transformed into plasmidless L. lactis MG1363 before a restriction endonuclease map was constructed. It was then fused with pUC19 to form pAJ02, which can replicate in Escherichia coli XLI-Blue as well as L. lactis MG1363. The plasmid was stably maintained in Lactococcus for more than 100 generations.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Biotechnology
DOI Number: https://doi.org/10.1080/10258140290010151
Keywords: Cecum; Escherichia coli; Lactococcus lactis; Shuttle vectorCecum; Escherichia coli; Lactococcus lactis; Shuttle vector
Depositing User: Ms. Azian Edawati Zakaria
Date Deposited: 25 Mar 2025 02:12
Last Modified: 25 Mar 2025 02:12
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/10258140290010151
URI: http://psasir.upm.edu.my/id/eprint/116287
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