Citation
Muhamad, Siti Nurfahirah and Md Akim, Abdah and Lim, Fang Lee and Karuppiah, Karmegam and Mohd Shabri, Nur Shabrina Azreen and How, Vivien
(2025)
Heat stress-induced heat shock protein 70 (HSP70) expressions among vulnerable populations in urban and rural areas Klang Valley, Malaysia.
Journal of Exposure Science and Environmental Epidemiology.
art. no. 4255.
pp. 1-9.
ISSN 1559-0631; eISSN: 1559-064X
(In Press)
Abstract
BACKGROUND: As climate change raises global temperatures, there remains a notable gap in understanding the body’s mechanisms of heat stress defense exhibited by Heat Shock Protein (HSP) within the populations.
OBJECTIVE: This study aims to investigate the expression level of HSP70 in response to indoor heat exposure among vulnerable populations in both urban and rural settings.
METHODS: A comparative cross-sectional was conducted among 108 participants from urban and rural areas in Klang Valley, Malaysia. The study included face-to-face interviews, indoor heat exposure monitoring, and thermal stress classification using the Universal Thermal Climate Index (UTCI). HSP70 gene and protein expressions were analyzed using reverse-transcription quantitative polymerase chain reaction (RT-qPCR) and HSP70 High Sensitivity Enzyme-linked Immunosorbent Assay (ELISA), respectively. RESULTS: Urban areas experienced signficantly higher UTCI heat exposure levels than rural areas (p < 0.001). In response to heat stress, vulnerable populations in urban areas exhibited higher HSP70 gene relative expression and HSP70 protein expression. A significant mean difference in the plasma HSP70 protein expression was observed between the two groups (p < 0.001). The linear mixed model (LMM) revealed a significant association between UTCI heat exposure levels and HSP70 gene and protein expression in both groups (p < 0.001).
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