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Heat stress-induced heat shock protein 70 (HSP70) expressions among vulnerable populations in urban and rural areas Klang Valley, Malaysia


Citation

Muhamad, Siti Nurfahirah and Md Akim, Abdah and Lim, Fang Lee and Karuppiah, Karmegam and Mohd Shabri, Nur Shabrina Azreen and How, Vivien (2025) Heat stress-induced heat shock protein 70 (HSP70) expressions among vulnerable populations in urban and rural areas Klang Valley, Malaysia. Journal of Exposure Science and Environmental Epidemiology. art. no. 4255. pp. 1-9. ISSN 1559-0631; eISSN: 1559-064X (In Press)

Abstract

BACKGROUND: As climate change raises global temperatures, there remains a notable gap in understanding the body’s mechanisms of heat stress defense exhibited by Heat Shock Protein (HSP) within the populations. OBJECTIVE: This study aims to investigate the expression level of HSP70 in response to indoor heat exposure among vulnerable populations in both urban and rural settings. METHODS: A comparative cross-sectional was conducted among 108 participants from urban and rural areas in Klang Valley, Malaysia. The study included face-to-face interviews, indoor heat exposure monitoring, and thermal stress classification using the Universal Thermal Climate Index (UTCI). HSP70 gene and protein expressions were analyzed using reverse-transcription quantitative polymerase chain reaction (RT-qPCR) and HSP70 High Sensitivity Enzyme-linked Immunosorbent Assay (ELISA), respectively. RESULTS: Urban areas experienced signficantly higher UTCI heat exposure levels than rural areas (p < 0.001). In response to heat stress, vulnerable populations in urban areas exhibited higher HSP70 gene relative expression and HSP70 protein expression. A significant mean difference in the plasma HSP70 protein expression was observed between the two groups (p < 0.001). The linear mixed model (LMM) revealed a significant association between UTCI heat exposure levels and HSP70 gene and protein expression in both groups (p < 0.001).


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Official URL or Download Paper: https://www.nature.com/articles/s41370-025-00764-4

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1038/s41370-025-00764-4
Publisher: Springer Nature
Keywords: Heat shock proteins 70 (HSP70); Heat stress; Indoor heat exposure; Universal thermal climate index (UTCI); Vulnerable population
Depositing User: Scopus
Date Deposited: 24 Mar 2025 03:15
Last Modified: 24 Mar 2025 03:15
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1038/s41370-025-00764-4
URI: http://psasir.upm.edu.my/id/eprint/116235
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