Citation
Asbi, B. Ali and Wei, L.S. and Steinberg, M.P.
(1989)
Effect of pH on the kinetics of soybean lipoxygenase‐1.
Journal of Food Science, 54 (6).
pp. 1594-1595.
ISSN 0022-1147; eISSN: 1750-3841
Abstract
The influence of pH on the kinetics of soybean lipoxygenase‐1 was examined. Lipoxygenase‐1 was stable when preincubated in the pH range from 3.2 to 9.2 but was irreversibly denatured at pH 3.0 and below. Double reciprocal plots for undenatured enzyme in the pH range 5.6 to 9.2 showed that the enzyme obeyed Michaelis‐Menten kinetics in this pH range. The Dixon plot (log Vmax/Km vs pH) indicated an active site of the free enzyme to be a histidine residue with a pK of about 7.3.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Universiti Pertanian Malaysia |
| DOI Number: | https://doi.org/10.1111/j.1365-2621.1989.tb05167.x |
| Publisher: | Wiley Blackwell |
| Keywords: | Soybean lipoxygenase-1; pH; Kinetics; Michaelis-Menten kinetics; Enzyme denaturation; Histidine residue; pK; Double reciprocal plots; Dixon plot |
| Depositing User: | Ms. Zaimah Saiful Yazan |
| Date Deposited: | 13 Mar 2025 02:44 |
| Last Modified: | 13 Mar 2025 02:44 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1111/j.1365-2621.1989.tb05167.x |
| URI: | http://psasir.upm.edu.my/id/eprint/115827 |
| Statistic Details: | View Download Statistic |
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