Citation
Abstract
Two areas in the chicken anemia virus (CAV) genome have high G:C content with secondary structures. These two G:C rich areas could not be sequenced with Perkin Elmer's Big Dye Terminator Cycle Sequencing Kit. Several modifications were carried out to solve the problem. Finally, a package of modified method was developed to sequence the high G:C areas. The result showed that the Perkin Elmer's Big Dye Terminator Cycle Sequencing Kit with the normal procedures are not suitable for sequencing the high G:C regions of the CAV genome. The present developed method made the Perkin Elmer's Kit useful for the first time to sequence the G:C rich hairpin structures of the CAV genome. The system may be useful to sequence all other G:C rich DNA templates.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Veterinary Medicine |
| DOI Number: | https://doi.org/10.1080/10258140290022310 |
| Publisher: | Taylor & Francis |
| Keywords: | Chicken anemia virus; Cloning; DNA templates; Sequencing G:C rich region |
| Depositing User: | Mohamad Jefri Mohamed Fauzi |
| Date Deposited: | 03 Mar 2025 02:56 |
| Last Modified: | 03 Mar 2025 02:56 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/10258140290022310 |
| URI: | http://psasir.upm.edu.my/id/eprint/115317 |
| Statistic Details: | View Download Statistic |
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