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Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum


Citation

Shuhaimi, M. and Ali, A.M. and Saleh, N.M. and Yazid, A.M. (2001) Cloning and sequence analysis of bile salt hydrolase (bsh) gene from Bifidobacterium longum. Biotechnology Letters, 23 (21). pp. 1775-1780. ISSN 0141-5492

Abstract

A pair of PCR primers for the rapid detection of bile salt hydrolase (bsh) gene from Bifidobacterium longum BB536 has been synthesised and have revealed the bsh gene of approx 970 bp in Bifidobacterium longum BB 536 but not in other species of bacteria tested. The bsh gene was cloned and sequenced showing a high similarity to bsh gene previously published. The resulting nucleotide sequence encodes a predicted protein of 317 amino acids, Mw = 35 kDa.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Food Science and Technology
DOI Number: https://doi.org/10.1023/A:1012400518207
Publisher: Springer Science and Business Media LLC
Keywords: Bifidobacterium longum; Bile salts; bsh gene; Cloning; PCR
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 26 Feb 2025 03:52
Last Modified: 26 Feb 2025 03:52
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1023/A:1012400518207
URI: http://psasir.upm.edu.my/id/eprint/115211
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