Citation
Lee, Kong H. and Lee, Pat M. and Siaw, Y.S. and Morihara, Kazuyuki
(1992)
Aspartame precursor synthesis in water miscible cosolvent catalysed by thermolysin.
Biotechnology Letters, 14 (12).
pp. 1159-1164.
ISSN 0141-5492; eISSN: 1573-6776
Abstract
The synthesis of the dipeptide N-benzyloxycarbonyl-L- aspartyl-phenylalanine methyl ester, aspartame precursor, catalysed by thermolysin in aqueous and aqueous methanolic solutions was studied. Thermolysin with concentration as low as 10 μM in 25% methanol can catalyse the synthetic reaction. The optimum methanol compositions at 4°C and 37°C were 50% and 25% respectively where an increase in peptide yield of 85% was obtained for both conditions as compared to that in water.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Food Science and Technology |
| DOI Number: | https://doi.org/10.1007/BF01027021 |
| Publisher: | Springer Science and Business Media LLC |
| Keywords: | Aspartame; Peptide synthesis; Thermolysin; Bacillus thermoproteolyticus |
| Depositing User: | Ms. Nuraida Ibrahim |
| Date Deposited: | 19 Feb 2025 07:44 |
| Last Modified: | 19 Feb 2025 07:44 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/BF01027021 |
| URI: | http://psasir.upm.edu.my/id/eprint/115092 |
| Statistic Details: | View Download Statistic |
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