Poly(Methyl Methacrylate) as a matrix for immobilization of lipase

Citation

Yunus, W. M. Z. Wan and Salleh, A. B. and Ismail, A. and Ampon, K. and Razak, C. N. A. and Basri, M. (1992) Poly(Methyl Methacrylate) as a matrix for immobilization of lipase. Applied Biochemistry and Biotechnology, 36 (2). pp. 97-105. ISSN 1559-0291; eISSN: 0273-2289

Abstract

Poly(methyl methacrylate) (PMMA) was found to be suitable for the immobilization of lipase from Candida rugosa. The best result based on hydrolytic activity was obtained by adsorption of the purified unbuffered enzyme solution onto PMMA beads without any modification of the beads. Prolonged exposure of the protein to the beads increased its adsorption but the expressed activity decreased after 1 h of exposure. The magnitude of the immobilized activity also varied with the size of the beads. Immobilization of the lipase shifted its optimal reaction temperature from 37 to 45°C. The immobilized enzyme is also more stable than the free enzyme in solution. The operational half-life of the immobilized lipase packed in a column and assayed in a closed system is 40 d.

Download File

Text
114984.pdf - Published Version
Restricted to Repository staff only
Download (404kB)

Official URL or Download Paper: https://link.springer.com/article/10.1007/BF029296...

Additional Metadata

Item Type:	Article
Divisions:	Faculty of Science and Environmental Studies Universiti Pertanian Malaysia
DOI Number:	https://doi.org/10.1007/BF02929689
Publisher:	Humana Press
Keywords:	Candida rugosa; Hydrolysis; Immobilization; Lipase; Poly(methyl methacrylate)
Depositing User:	Mohamad Jefri Mohamed Fauzi
Date Deposited:	13 Feb 2025 06:50
Last Modified:	13 Feb 2025 06:50
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/BF02929689
URI:	http://psasir.upm.edu.my/id/eprint/114984
Statistic Details:	View Download Statistic

Actions (login required)

View Item