Citation
Ampon, Kamaruzaman
(1992)
Distribution of an enzyme in porous polymer beads.
Journal of Chemical Technology & Biotechnology, 55 (2).
pp. 185-190.
ISSN 0268-2575; eISSN: 1097-4660
Abstract
Trypsin has been immobilized by adsorption onto Amberlite XAD‐7 beads. The Michaelis constant (Km) of the enzyme was increased about sevenfold following the immobilization. Its rate of penetration into the porous beads was determined by staining the beads, which had been split, with naphthol blue black. The extent of diffusional rate limitation of immobilized trypsin was related to the penetration depth of the enzyme into the beads. This can be controlled by manipulating the conditions during the preparation of the immobilized enzyme. Copyright © 1992 Society of Chemical Industry
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences |
| DOI Number: | https://doi.org/10.1002/jctb.280550213 |
| Keywords: | Immobilized trypsin; Porous support; Protein staining |
| Depositing User: | Ms. Azian Edawati Zakaria |
| Date Deposited: | 28 Jan 2025 06:55 |
| Last Modified: | 28 Jan 2025 06:55 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1002/jctb.280550213 |
| URI: | http://psasir.upm.edu.my/id/eprint/114746 |
| Statistic Details: | View Download Statistic |
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