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Isolation and screening of an extracellular organic solvent-tolerant protease producer


Citation

Geok, Lee Poh and Nyonya, Che and Razak, Abdul and Abd Rahman, Raja Noor Zaliha (2003) Isolation and screening of an extracellular organic solvent-tolerant protease producer. Biochemical Engineering Journal, 13 (1). pp. 73-77. ISSN 1369-703X; eISSN: 1369-703X

Abstract

An extracellular organic solvent-tolerant protease producer has been successfully isolated out of 11 isolates of benzene-toluene-xylene-ethylbenzene (BTEX) tolerant bacteria. This organic solvent-tolerant microorganism was found to be a polycyclic-aromatic-hydrocarbons (PAHs) degrader and identified as Pseudomonas aeruginosa strain K. It was selected based on the stability of its proteolytic enzyme in the presence of various organic solvents. P. aeruginosa strain K protease tolerated up to at least 50% (v/v) of benzene, n-hexane, 1-decanol, isooctane and n-hexadecane and was also stable in the presence of 25% (v/v) n-decane and n-dodecane. This enzyme strain K was activated 2.5, 1.5 and 1.2 times by 75% (v/v) of 1-decanol, isooctane and n-dodecane, respectively. This organic solvent stable protease could be used as a biocatalyst for enzymatic synthesis in the presence of organic solvents.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Science and Environmental Studies
DOI Number: https://doi.org/10.1016/S1369-703X(02)00137-7
Publisher: Elsevier Science B.V.
Keywords: Organic solvent-tolerant; Protease; Pseudomonas aeruginosa
Depositing User: Ms. Zaimah Saiful Yazan
Date Deposited: 05 Feb 2025 03:55
Last Modified: 05 Feb 2025 03:55
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/S1369-703X(02)00137-7
URI: http://psasir.upm.edu.my/id/eprint/114458
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