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Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells


Citation

Inayat-Hussain, Salmaan H. and Osman, Annuar B. and Din, Laily B. and Ali, Abdul M. (1999) Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells. FEBS Letters, 456 (3). pp. 379-383. ISSN 0014-5793; eISSN: 0014-5793

Abstract

Goniothalamin, a plant styrylpyrone derivative isolated from Goniothalamus andersonii, induced apoptosis in Jurkat T-cells as assessed by the externalisation of phosphatidylserine. Immunoblotting showed processing of caspases-3 and -7 with the appearance of their catalytically active large subunits of 17 and 19 kDa, respectively. Activation of these caspases was further evidenced by detection of poly(ADP-ribose) polymerase cleavage (PARP). Pre-treatment with the caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp fluoromethyl ketone (Z-VAD.FMK) blocked apoptosis and the resultant cleavage of these caspases and PARP. Our results demonstrate that activation of at least two effector caspases is a key feature of goniothalamin-induced apoptosis in Jurkat T-cells.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1016/S0014-5793(99)00984-9
Publisher: Federation of European Biochemical Societies
Keywords: Apoptosis; Caspase-3; Caspase-7; Goniothalamin; Jurkat T-cell
Depositing User: Ms. Zaimah Saiful Yazan
Date Deposited: 15 Jan 2025 07:40
Last Modified: 15 Jan 2025 07:40
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/S0014-5793(99)00984-9
URI: http://psasir.upm.edu.my/id/eprint/114448
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