Citation
Augustin, M.A. and Ghazali, Hasanah M. and Hashim, Hasimah
(1985)
Polyphenoloxidase from guava (Psidium guajava L.).
Journal of the Science of Food and Agriculture, 36 (12).
pp. 1259-1265.
ISSN 0022-5142; eISSN: 1097-0010
Abstract
Polyphenoloxidase (PPO) was isolated from guava (Psidium guajava L., cv. Kampuchea). Purification by acetone precipitation and dialysis of the Amicon concentrate resulted in 4.9‐fold and 17.2‐fold purification of the dialysed crude extract respectively. Polyacrylamide gel electrophoresis indicated that PPO had a molecular weight in the region of 120000–140000. The pH optimum of the enzyme was found to be 7.2. Heat inactivation studies showed that heating for 1.1, 3.5, and 10.1 min at 75, 65 and 55°C respectively caused a 50% loss in enzymic activity. The enzyme‐catalysed browning reaction was significantly inhibited in the presence of sodium metabisulphite, L‐cysteine hydrochloride and ascorbic acid.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Agriculture and Food Sciences Universiti Pertanian Malaysia |
| DOI Number: | https://doi.org/10.1002/jsfa.2740361209 |
| Publisher: | John Wiley & Sons |
| Keywords: | Guava; Polyphenoloxidase; Psidium guajava |
| Depositing User: | Ms. Zaimah Saiful Yazan |
| Date Deposited: | 14 Jan 2025 02:57 |
| Last Modified: | 14 Jan 2025 02:57 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1002/jsfa.2740361209 |
| URI: | http://psasir.upm.edu.my/id/eprint/114332 |
| Statistic Details: | View Download Statistic |
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