Citation
Yahaya, H. and Cheah, Y.K. and Chee, H.Y. and Than, L.T.L.
(2024)
Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection.
European Journal of Clinical Microbiology and Infectious Diseases, 43.
pp. 2211-2214.
ISSN 0934-9723; eISSN: 1435-4373
Abstract
Purpose: Loop-mediated isothermal amplification (LAMP) is a simple and rapid nucleic acid method for DNA amplification at a constant temperature. The “gold standard” culture method for yeast detection, has low sensitivity with severe consequences, increasing morbidity and mortality rates. Here, we report the development of a LAMP method for the specific detection of C. glabrata. Methodology: The specific LAMP primers for C. glabrata detection were designed and evaluated. Results: The LAMP assay accurately detected C. glabrata with no cross-reactivity with other Candida species. Conclusion: The developed molecular method would be a promising tool in the management of invasive candidiasis.
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Official URL or Download Paper: https://link.springer.com/article/10.1007/s10096-0...
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Medicine and Health Science |
| DOI Number: | https://doi.org/10.1007/s10096-024-04934-4 |
| Publisher: | Springer Science and Business Media Deutschland |
| Keywords: | LAMP; PCR; C. glabrata; Culture; Invasive candidiasis |
| Depositing User: | Ms. Nur Faseha Mohd Kadim |
| Date Deposited: | 08 Jan 2025 04:44 |
| Last Modified: | 08 Jan 2025 04:44 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s10096-024-04934-4 |
| URI: | http://psasir.upm.edu.my/id/eprint/114237 |
| Statistic Details: | View Download Statistic |
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