UPM Institutional Repository

Antibacterial activity of free and liposome-encapsulated Tylosin against planktonic and biofilm forms of Corynebacterium pseudotuberculosis isolated from goats


Citation

Saddiqi, Mohammad Ehsan and Kadir, Arifah Abdul (2024) Antibacterial activity of free and liposome-encapsulated Tylosin against planktonic and biofilm forms of Corynebacterium pseudotuberculosis isolated from goats. Acta Veterinaria Eurasia, 50 (2). pp. 119-127. ISSN 2619-905X; eISSN: 2619-905X

Abstract

Corynebacterium pseudotuberculosis causes infection in goats, sheep, cattle, and humans. The disease imposing huge economic losses such as reducing milk, meat, and wool production and downgrading and condemnation of affected carcass to goat-rearing industry. Despite all its devastating effects, no effective antimicrobial was suggested for the treatment of this pathogen due to its highly resistant nature. Development and utilization of nanoparticles has paved the way to revive the efficacy of conventional antibiotics. Encapsulation of antibiotics within liposome as a nanoparticle may present a potential approach for successful treatment of resistant bacterial infections. The aim of this research was therefore to evaluate the antibacterial activity of free and liposome-encapsulating tylosin against Corynebacterium pseudotuberculosis. The minimum inhibitory concentration of free and liposome-encapsulated tylosin was determined by microbroth dilution and resazurin colorimetric-based methods. The antibiofilm activity of free and liposomal tylosin was assessed by crystal violet and resazurin techniques. Acridine orange/ethidium bromide staining assay was performed to assess the effect of free and liposomal tylosin on pre-formed biofilm of Corynebacterium pseudotuberculosis. The findings demonstrated minimum inhibitory concentration value of 1 μg/mL and 32 μg/mL for free and liposomal tylosin, respectively. The biofilm inhibitory data showed that free tylosin inhibited the formation of Corynebacterium pseudotuberculosis at 2 μg/mL, while this concentration was 64 μg/mL for liposomal tylosin. The antibiofilm concentration was 1024 μg/mL and 2048 μg/mL for liposome-encapsulated and free tylosin, respectively. It can be concluded that liposome as a nanocarrier can offer an excellent system of drug delivery for enhancing the efficacy of antibiotics against resistant form of Corynebacterium pseudotuberculosis.


Download File

[img] Text
113554.pdf - Published Version
Available under License Creative Commons Attribution Non-commercial.

Download (2MB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.5152/actavet.2024.23070
Publisher: Istanbul University
Keywords: Biofilm; Corynebacterium pseudotuberculosis; Liposome; Planktonic; Tylosin
Depositing User: Mr. Mohamad Syahrul Nizam Md Ishak
Date Deposited: 26 Nov 2024 04:18
Last Modified: 26 Nov 2024 04:18
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5152/actavet.2024.23070
URI: http://psasir.upm.edu.my/id/eprint/113554
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item