Citation
Fayyaz, A. and Asbi, B.A. and Ghazali, H.M. and Che Man, Y.B. and Jinap, S.
(1994)
Purification and molecular properties of papaya pectinesterase.
Food Chemistry, 49 (4).
pp. 373-378.
ISSN 0308-8146; eISSN: 1873-7072
Abstract
Pectinesterase (EC 3.1.1.11) was extracted and purified from papaya (Carica papaya L. var. exotica). The procedure adopted for purification resulted in an approximate 250-fold purification (784 units/mg protein) with a 45% recovery of the pectinesterase activity. The enzyme was eluted in a single peak after CmdashSephadex and Sephadex G-100 chromatography. The purified enzyme had a uniform specific activity throughout the final chromatographic peak. The enzyme preparation was confirmed to be of homogeneous state by gel filtration and nondashdenaturing polyacrylamide gel electrophoresis and it has a molecular weight of approximately 32 000 Da.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Food Science and Biotechnology Universiti Pertanian Malaysia |
| DOI Number: | https://doi.org/10.1016/0308-8146(94)90007-8 |
| Publisher: | Elsevier |
| Keywords: | Pectinesterase; Carica papaya L.; purification; molecular properties |
| Depositing User: | Ms. Zaimah Saiful Yazan |
| Date Deposited: | 22 Jan 2025 02:06 |
| Last Modified: | 22 Jan 2025 02:06 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/0308-8146(94)90007-8 |
| URI: | http://psasir.upm.edu.my/id/eprint/113113 |
| Statistic Details: | View Download Statistic |
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