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Nucleotide sequence of the haemagglutinin-neuraminidase (HN) gene of a Malaysian heat resistant viscerotropic-velogenic Newcastle disease virus (NDV) strain AF2240


Citation

Tan, W. S. and Lau, C. H. and Ng, B. K. and Ibrahim, A. L. and Yusoff, K. (1995) Nucleotide sequence of the haemagglutinin-neuraminidase (HN) gene of a Malaysian heat resistant viscerotropic-velogenic Newcastle disease virus (NDV) strain AF2240. DNA Sequence, 6 (1). pp. 47-50. ISSN 1042-5179

Abstract

The nucleotide sequence of the haemagglutinin-neuraminidase (HN) gene of Newcastle disease virus (NDV) viscerotropic-velogenic strain AF2240 was determined by direct RNA sequencing and by sequencing RT-PCR products. It encodes a single open reading frame of 581 amino acids with a calculated Mr of 63.8 kDa. The predicted sequence contains five asparagine giycosylation sites. Comparison of the AF2240 HN protein sequence with 13 other previously published sequences showed 88% homology. This HN protein is unique because it lacked the Arg 403 residue which is present in all of the other strains and cannot be grouped under the proposed three size classes of HN proteins in NDV.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Universiti Pertanian Malaysia
DOI Number: https://doi.org/10.3109/10425179509074699
Publisher: Informa UK Limited
Keywords: HN gene sequence; Viscerotropic-velogenic NDV strain
Depositing User: Ms. Zaimah Saiful Yazan
Date Deposited: 13 Jan 2025 07:42
Last Modified: 13 Jan 2025 07:42
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3109/10425179509074699
URI: http://psasir.upm.edu.my/id/eprint/112719
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