Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria

Citation

Shuhaimi, M. and Ali, A.M. and Saleh, N.M. and Yazid, A.M. (2001) Utilisation of enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR to fingerprint the genomes of Bifidobacterium isolates and other probiotic bacteria. Biotechnology Letters, 23 (9). pp. 731-736. ISSN 0141-5492; eISSN: 1573-6776

Abstract

Enterobacterial repetitive intergenic consensus based on PCR (ERIC-PCR) was used to generate DNA fingerprints for bifidobacteria and other probiotic bacteria. Two primers (ERIC 1R and ERIC 2) used in ERIC-PCR revealed that all of the probiotic bacteria tested possess enterobacterial repetitive intergenic consensus sequences with the PCR products ranging from 250 bp to 5000 bp. The bacterial strains can be differentiated by comparing fingerprint patterns. The dendrogram of the fingerprints revealed that most of the bifidobacterial wild type strains fell into one cluster at similarity level of approximately 79%.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Biotechnology and Biomolecular Sciences Faculty of Food Science and Technology
DOI Number:	https://doi.org/10.1023/a:1010355003674
Publisher:	Springer
Notes:	Bifidobacteria; DNA fingerprints; Genome probiotic bacteria
Depositing User:	Ms. Zaimah Saiful Yazan
Date Deposited:	06 Mar 2025 00:58
Last Modified:	06 Mar 2025 00:58
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1023/a:1010355003674
URI:	http://psasir.upm.edu.my/id/eprint/112670
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