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Effect of promoter strength and signal sequence on the periplasmic expression of human interferon-α2b in Escherichia coli


Citation

Ramanan, Ramakrishnan Nagasundara and Wong, Bee Tik and Memari, Hamid Rajabi and Azaman, Siti Nor Ani and Tau, Chuan Ling and Tey, Beng Ti and Mohd Lila, Mohd Azmi and Abdullah, Mohd Puad and Abdul Rahim, Raha and Ariff, Arbakariya (2010) Effect of promoter strength and signal sequence on the periplasmic expression of human interferon-α2b in Escherichia coli. African Journal of Biotechnology, 9 (3). art. no. E05979530638. pp. 285-292. ISSN 1684–5315

Abstract

Two plasmids, pFLAG-ATS and pET 26b(+), were studied for the periplasmic expression of recombinant human interferon-α2b (IFN-α2b) in Escherichia coli. The pFLAG-ATS contains ompA signal sequence and tac promoter while pET 26b(+) contains pelB signal sequence and T7lac promoter. It was observed that periplasmic expression of IFN-α2b from pET 26b(+) was around 3000 times higher than pFLAG-ATS. Difference in the expression level was attributed to the difference in the promoters and the signal sequences. In silico analysis of mRNA secondary structures were analyzed using Vienna RNA package and MFOLD. The resultssuggested that the increase of expression would mainly due to the difference in the translation initiation associated with secondary structure of mRNA transcribed by both plasmids.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
Publisher: Academic Journals
Keywords: Interferon-α2b; Periplasm; Signal sequence; mRNA secondary structure; Escherichia coli
Depositing User: Anas Yahaya
Date Deposited: 29 Mar 2011 08:42
Last Modified: 14 Nov 2017 02:48
URI: http://psasir.upm.edu.my/id/eprint/11248
Statistic Details: View Download Statistic

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