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Isolation of microsatellite markers in mungbean, Vigna radiata


Citation

Kumar, S. V. and Tan, S. G. and Quah, S. C. and Yusoff, K. (2002) Isolation of microsatellite markers in mungbean, Vigna radiata. Molecular Ecology Notes, 2 (2). pp. 96-98. ISSN 1471-8278

Abstract

A simple and rapid method for isolating microsatellite loci in mungbean, Vigna radiata,based on the 5′′′′-anchored polymerase chain reaction technique revealed 23 microsatelliteloci and six cryptically simple sequence repeats. We report on the characterization of sevenpolymorphic microsatellite loci in V. radiata. The number of alleles per locus ranged from2 to 5 while the observed heterozygosity ranged from 0 to 0.9048. These markers shouldprove useful as tools for detecting genetic variation in mungbean varieties for germplasmmanagement and crossbreeding purposes.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1046/j.1471-8286.2002.00158.x
Publisher: Wiley
Keywords: Microsatellites; Mungbean; Polymorphism; Vigna
Depositing User: Ms. Zaimah Saiful Yazan
Date Deposited: 03 Mar 2025 07:51
Last Modified: 03 Mar 2025 07:51
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1046/j.1471-8286.2002.00158.x
URI: http://psasir.upm.edu.my/id/eprint/111737
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