Safety evaluation of secretome proteins from paenibacillus polymyxa kp10 and lactococcus lactis gh1 as the potential antimicrobial therapeutic agent

Citation

Zainal Baharin, Nurul Hana and Khairil Mokhtar, Nur Fadhilah and Mohd Desa, Mohd Nasir and Dzaraly, Nurul Diana and Muthanna, Abdulrahman and Mohd Hashim, Amalia and Lani, Mohd Nizam and Mustafa, Shuhaimi (2023) Safety evaluation of secretome proteins from paenibacillus polymyxa kp10 and lactococcus lactis gh1 as the potential antimicrobial therapeutic agent. Halalsphere, 3 (2). pp. 1-6. ISSN 2773-6040

Abstract

The secretome proteins of Paenibacillus polymyxa Kp10 and Lactococcus lactis Gh1 were previously found to have the potential for controlling the antibiotic-resistant pathogen. Therefore, their safety evaluation to ensure consumer health is deemed important. This study was to evaluate the toxicity effect of both secretome proteins in human cells against Medical Research Council cell strain 5 (MRC5). Then, their antibacterial activities were tested in human serum to assess the stability of both secretome proteins. The results showed no cytotoxic effects of either secretome protein when MRC5 cells were treated up to the determined concentrations. Therefore, no IC50 was determined. In addition, human serum did not affect the antibacterial activity of both secretome proteins against Methicillin-resistant Staphylococcus aureus (MRSA) and Vancomycin-resistant Enterococcus (VRE). In conclusion, both secretome proteins are potentially safe for future development as a potential therapeutic agent.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Biotechnology and Biomolecular Sciences Faculty of Medicine and Health Science Halal Products Research Institute
DOI Number:	https://doi.org/10.31436/hs.v3i2.62
Publisher:	IIUM Press
Keywords:	Secretome proteins, Paenibacillus polymyxa Kp10, Lactococcus lactis Gh1, Safety evaluation and Antibiotic resistance; Good Health and Well-being
Depositing User:	Ms. Che Wa Zakaria
Date Deposited:	10 Oct 2024 07:14
Last Modified:	10 Oct 2024 07:14
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.31436/hs.v3i2.62
URI:	http://psasir.upm.edu.my/id/eprint/108871
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