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The molecular approaches and challenges of Streptococcus pneumoniae serotyping for epidemiological surveillance in the vaccine era


Citation

Abdul Rahman, Nurul Asyikin and Mohd Desa, Mohd Nasir and Masri, Siti Norbaya and Taib, Niazlin Mohd and Sulaiman, Nurshahira and Hazman, Hazmin and John, James (2023) The molecular approaches and challenges of Streptococcus pneumoniae serotyping for epidemiological surveillance in the vaccine era. Polish Journal of Microbiology, 72 (2). pp. 103-115. ISSN 1733-1331; eISSN: 2544-4646

Abstract

Streptococcus pneumoniae (pneumococcus) belongs to the Gram-positive coccus category. This bacterium typically colonises the nasopharyngeal region of healthy individuals. It comprises an extremely distinct polysaccharide capsule adding to virulence, allowing the bacteria to elude the immune defence mechanisms. Consequently, it might trigger aggressive conditions like septicaemia and meningitis in immunocompromised or older individuals; moreover, children below five years of age, are at risk of morbidity and mortality. Studies have found 101 S. pneumoniae capsular serotypes, of which several correlate with clinical and carriage isolates with distinct disease aggressiveness. The introduction of pneumococcal conjugate vaccines (PCV) targets the most common disease-associated serotypes. Nevertheless, vaccine selection pressure leads to replacement of the formerly dominant vaccine serotypes (VTs) by non-vaccine types (NVTs). Therefore, serotyping must be conducted for epidemiological surveillance and vaccine assessment. Serotyping can be performed using numerous techniques either by the conventional antisera-based (Quellung and latex agglutination) or molecular based approaches (sequetyping, multiplex PCR, realtime PCR and PCR-RFLP). A cost-effective and practical approach must be used to enhance serotyping accuracy to monitor the prevalence of VTs and NVTs. Therefore, dependable pneumococcal serotyping techniques are essential to precisely monitor virulent lineages, NVT emergence, and genetic associations of isolates. This review discusses the principles, the associated benefits and drawbacks for the respective available conventional and molecular approaches, and potentially the whole genome sequencing (WGS) to be directed for future exploration.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.33073/pjm-2023-023
Publisher: Polish Society of Microbiologists
Keywords: Streptococcus pneumoniae; Serotyping; PCR; Sequetyping; PCR-RFLP; WGS; Good health and well-being
Depositing User: Ms. Che Wa Zakaria
Date Deposited: 11 Oct 2024 08:57
Last Modified: 11 Oct 2024 08:57
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.33073/pjm-2023-023
URI: http://psasir.upm.edu.my/id/eprint/108735
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