UPM Institutional Repository

Immunogenicity and efficacy of a feed-based bivalent vaccine against streptococcosis and motile Aeromonad septicemia in red hybrid tilapia (Oreochromis sp.)


Citation

Mohd Ali, Nur Shidaa and Saad, Mohd Zamri and Azmai, Mohammad Noor Amal and Salleh, Annas and Mohamed Zulperi, Zarirah and Manchanayake, Tilusha and Zahaludin, Muhammad Amir Danial and Basri, Lukman and Mohamad, Aslah and Md Yasin, Ina Salwany (2023) Immunogenicity and efficacy of a feed-based bivalent vaccine against streptococcosis and motile Aeromonad septicemia in red hybrid tilapia (Oreochromis sp.). Animals, 13 (8). art. no. 1346. pp. 1-20. ISSN 2076-2615; ESSN: 2076-2615

Abstract

Streptococcosis and motile Aeromonad septicemia (MAS) are the main bacterial diseases in tilapia culture worldwide, causing significant economic losses. Vaccination is an effective method of preventing diseases and contributes to economic sustainability. This study investigated the immuno-protective efficacy of a newly developed feed-based bivalent vaccine against streptococcosis and MAS in red hybrid tilapia. The feed-based bivalent vaccine pellet was developed by incorporating the formalin-killed S. agalactiae and A. hydrophila antigens into a commercial feed pellet with palm oil as the adjuvant. The bivalent vaccine was subjected to feed quality analyses. For immunological analyses, 900 fish (12.94 ± 0.46 g) were divided into two treatment groups in triplicate. Fish in Group 1 were unvaccinated (control), while those in Group 2 were vaccinated with the bivalent vaccine. The bivalent vaccine was delivered orally at 5 of the fishs body weight for three consecutive days on week 0, followed by boosters on weeks 2 and 6. Lysozyme and enzyme-linked immunosorbent assays (ELISAs) on serum, gut lavage, and skin mucus were performed every week for 16 weeks. Lysozyme activity in vaccinated fish was significantly (p ‰ 0.05) higher than in unvaccinated fish following vaccination. Similarly, the IgM antibody levels of vaccinated fish were significantly (p ‰ 0.05) higher after vaccination. The bivalent vaccine provided high protective efficacy against S. agalactiae (80.00 ± 10.00) and A. hydrophila (90.00 ± 10.00) and partial cross-protective efficacy against S. iniae (63.33 ± 5.77) and A. veronii (60.00 ± 10.00). During the challenge test, fewer clinical and gross lesions were observed in vaccinated fish compared with unvaccinated fish. Histopathological assessment showed less severe pathological changes in selected organs than the unvaccinated fish. This study showed that vaccination with a feed-based bivalent vaccine improves immunological responses in red hybrid tilapia, and thus protects against streptococcosis and MAS.


Download File

Full text not available from this repository.
Official URL or Download Paper: https://www.mdpi.com/2076-2615/13/8/1346

Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Science
Faculty of Veterinary Medicine
Institute of Bioscience
DOI Number: https://doi.org/10.3390/ani13081346
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
Keywords: Red hybrid tilapia; Streptococcosis; Motile Aeromonad septicemia; Feed-based bivalent vaccine; Immuno-protective
Depositing User: Ms. Zaimah Saiful Yazan
Date Deposited: 11 Sep 2024 02:30
Last Modified: 11 Sep 2024 02:30
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/ani13081346
URI: http://psasir.upm.edu.my/id/eprint/108272
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item