Citation
Abstract
The present study determined the in-situ antibacterial effect of cationic kenaf seed peptides mixture (KSPM) in whole milk and further comprehensively elucidated the mechanisms underlying the bactericidal action against gram-positive and gram-negative bacteria. The application experiments in artificially contaminated milk showed that KSPM at 0.5 and 1 mg/mL displayed a stable and strong bactericidal activity within 1“6 h of treatment and lasted up to two weeks storage at room and refrigeration conditions. The in-vitro mechanistic study showed that the KSPM underwent conformational changes from random coil to ±-helix and ²-sheet in the membrane-mimetic environment. The KSPM exerted its inhibitory effect in a concentration and time dependent manner through cell membrane permeabilisation associated with membrane-disrupting effects, which is characterised by the cellular material leakage. The in-silico study revealed that the peptides efficiently interacted with bacterial peptidoglycan via hydrogen bonding, electrostatic interactions and hydrophobic forces. The peptide RALPSEHNK interacted with gram-positive and gram-negative bacterial phospholipid phosphate head group mainly through electrostatic interaction. The current work indicated that KSPM exerts its bactericidal effect by membrane-targeted mechanism and it would be a promising natural candidate to control bacterial contamination in milk and dairy-based products.
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Additional Metadata
Item Type: | Article |
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Divisions: | Faculty of Food Science and Technology Faculty of Medicine and Health Science |
DOI Number: | https://doi.org/10.1016/j.lwt.2023.114757 |
Publisher: | Elsevier |
Keywords: | Milk preservation; Bactericidal; Membrane-active peptide; Molecular docking; Molecular dynamics simulation; Responsible consumption; Production; Agriculture; Food Security; SDG 2: Zero Hunger |
Depositing User: | Ms. Nur Aina Ahmad Mustafa |
Date Deposited: | 12 Sep 2024 04:06 |
Last Modified: | 12 Sep 2024 04:06 |
Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.lwt.2023.114757 |
URI: | http://psasir.upm.edu.my/id/eprint/107651 |
Statistic Details: | View Download Statistic |
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