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Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation


Citation

Arulrajah, Brisha and Qoms, Mohammed S. and Muhialdin, Belal J. and Meor Hussin, Anis Shobirin and Hasan, Hanan and Zarei, Mohammad and Chau, De-Ming and Ramasamy, Rajesh and Saari, Nazamid (2023) Elucidating the mechanisms underlying the action of kenaf seed peptides mixture against gram-positive and gram-negative bacteria and its efficacy in whole milk preservation. LWT - Food Science and Technology, 181. art. no. 114757. ISSN 0023-6438

Abstract

The present study determined the in-situ antibacterial effect of cationic kenaf seed peptides mixture (KSPM) in whole milk and further comprehensively elucidated the mechanisms underlying the bactericidal action against gram-positive and gram-negative bacteria. The application experiments in artificially contaminated milk showed that KSPM at 0.5 and 1 mg/mL displayed a stable and strong bactericidal activity within 1“6 h of treatment and lasted up to two weeks storage at room and refrigeration conditions. The in-vitro mechanistic study showed that the KSPM underwent conformational changes from random coil to ±-helix and ²-sheet in the membrane-mimetic environment. The KSPM exerted its inhibitory effect in a concentration and time dependent manner through cell membrane permeabilisation associated with membrane-disrupting effects, which is characterised by the cellular material leakage. The in-silico study revealed that the peptides efficiently interacted with bacterial peptidoglycan via hydrogen bonding, electrostatic interactions and hydrophobic forces. The peptide RALPSEHNK interacted with gram-positive and gram-negative bacterial phospholipid phosphate head group mainly through electrostatic interaction. The current work indicated that KSPM exerts its bactericidal effect by membrane-targeted mechanism and it would be a promising natural candidate to control bacterial contamination in milk and dairy-based products.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1016/j.lwt.2023.114757
Publisher: Elsevier
Keywords: Milk preservation; Bactericidal; Membrane-active peptide; Molecular docking; Molecular dynamics simulation; Responsible consumption; Production; Agriculture; Food Security; SDG 2: Zero Hunger
Depositing User: Ms. Nur Aina Ahmad Mustafa
Date Deposited: 12 Sep 2024 04:06
Last Modified: 12 Sep 2024 04:06
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.lwt.2023.114757
URI: http://psasir.upm.edu.my/id/eprint/107651
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