Citation
Addisu Biffa, Melaku
(2003)
Conservation and Diversity of Hempedu Bumi (Andrographis Paniculata Nees) Germplasm in Malaysia.
Masters thesis, Universiti Putra Malaysia.
Abstract
Andrographis paniculata, a member of Accantaceae family, is a soft wooded
herbaceous plant having multiple uses in traditional as well as pharmacological
(modern) medicine. Collection was carried out on A. paniculat a germplasm from
Selangor, Negeri Sembilan and Perak states of Peninsular Malaysia. The
objectives of the study were to conserve A. paniculata germplasm from different
agroecological conditions of Peninsular Malaysia, to analyze variability and
rationalize the germplasm accessions using agro-morphological traits and to
evaluate the germ plasm and thereby identify promising genotypes.
As noticed during the collection activity, the habitat preference of the plant was
wide. The ranges of habitats include neglected fields invaded with noxious
weeds, rocky and poor soil conditions and backyard of houses in villages. Of the total 38 accessions collected, 26 were evaluated in the field using
Randomized Complete Block Design in three replications. The coefficient of
variation values for most of the quantitative morphological characters were
generally moderate. Moreover, the qualitative traits such as mature and
immature leaf color, leaf shape, plant growth habit and stem color showed the
presence of d ifferent types within the germplasm studied.
Cluster analYSis done on 26 accessions showed the presence of four d istinct
groups. However, this grouping pattern did not show parallelism between the
geographical and genetic diversity. The result generally showed the existence of
similar genotypes in all of the three states considered u nder this study.
Principal component analysis showed the first two axes represent 66.67% of the
total variation that identified the importance of flowering date, shoot fresh weight,
root fresh weight, leaf dry weight, stem dry weight, root dry weight and root
volume towards genetic divergence. The first axis was able to differentiate the
first two groups from the 3rd and 4th groups from the cluster analysis.
Heritability values revealed high to moderate for flowering date, shoot fresh
weight, root dry weight, root fresh weight, branch length, root volume, plant
height, leaf dry weight and leaf width.
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