Citation
Abstract
Background Severe leptospirosis is challenging as it could evolve rapidly and potentially fatal if appropri-ate management is not performed. An understanding of the progression and pathophysiology of Leptospira infection is important to determine the early changes that could be potentially used to predict the severe occurrence of leptospirosis. This study aimed to understand the kinetics pathogenesis of Leptospira interrogans strain HP358 in the hamster model and iden-tify the early parameters that could be used as biomarkers to predict severe leptospirosis. Methodology/Principal findings Male Syrian hamsters were infected with Leptospira interrogans strain HP358 and eutha-nized after 24 hours, 3, 4, 5, 6 and 7 days post-infection. Blood, lungs, liver and kidneys were collected for leptospiral detection, haematology, serum biochemistry and differential expression of pro-and anti-inflammatory markers. Macroscopic and microscopic organ damages were investigated. Leptospira interrogans strain HP358 was highly pathogenic and killed hamsters within 6–7 days post-infection. Pulmonary haemorrhage and blood ves-sel congestion in organs were noticed as the earliest pathological changes. The damages in organs and changes in biochemistry value were preceded by changes in haematology and immune gene expression. Conclusion/Significance This study deciphered haemorrhage as the earliest manifestation of severe leptospirosis and high levels of IL-1β, CXCL10/IP-10, CCL3/MIP-α, neutrophils and low levels of lymphocytes and platelets serve as a cumulative panel of biomarkers in severe leptospirosis.
Download File
Full text not available from this repository.
Official URL or Download Paper: https://journals.plos.org/plosntds/article?id=10.1...
|
Additional Metadata
Item Type: | Article |
---|---|
Divisions: | Faculty of Medicine and Health Science Faculty of Veterinary Medicine Institute of Bioscience |
DOI Number: | https://doi.org/10.1371/journal.pntd.0010409 |
Publisher: | Public Library of Science |
Keywords: | Leptospirosis; Early diagnosis; Biomarker identification; Pathogenesis; Hamster model; Good health and well-being |
Depositing User: | Mr. Mohamad Syahrul Nizam Md Ishak |
Date Deposited: | 30 Jun 2024 06:11 |
Last Modified: | 30 Jun 2024 06:11 |
Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1371/journal.pntd.0010409 |
URI: | http://psasir.upm.edu.my/id/eprint/102955 |
Statistic Details: | View Download Statistic |
Actions (login required)
View Item |