Citation
Abstract
Objective: The degradation activity of two bacteriophages UPMK_1 and UPMK_2 against methicillin-resistant Staphylococcus aureus phages were examined using gel zymography. Methods: The analysis was done using BLASTP to detect peptides catalytic domains. Many peptides that are related to several phage proteins were revealed. Results: UPMK_1 and UPMK_2 custom sequence database were used for peptide identification. The biofilm-degrading proteins in the bacteriophage UPMK_2 revealed the same lytic activity towards polysaccharide intercellular adhesin-dependent and independent of Methicillin-resistant Staphylococcus aureus (MRSA) biofilm producers in comparison to UPMK_1, which had lytic activity restricted solely to its host. Conclusion: Both bacteriophage enzymes were involved in MRSA biofilm degradation during phage infection and they have promising enzybiotics properties against MRSA biofilm formation.
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Official URL or Download Paper: https://link.springer.com/article/10.1007/s10529-0...
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Agriculture Faculty of Biotechnology and Biomolecular Sciences Faculty of Medicine and Health Science Institute of Bioscience |
| DOI Number: | https://doi.org/10.1007/s10529-022-03229-y |
| Publisher: | Springer |
| Keywords: | Bacteriophages; Biofilm degradation; MRSA, phage enzymes; Zymogram |
| Depositing User: | Ms. Nuraida Ibrahim |
| Date Deposited: | 21 Nov 2024 04:51 |
| Last Modified: | 21 Nov 2024 04:51 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s10529-022-03229-y |
| URI: | http://psasir.upm.edu.my/id/eprint/102935 |
| Statistic Details: | View Download Statistic |
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