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Phytochemicals and antioxidant activities of conventionally propagated nodal segment and in vitro-induced callus of Bougainvillea glabra Choisy using different solvents


Citation

Nasrat, Mohammad Nasim and Sakimin, Siti Zaharah and Hakiman, Mansor (2022) Phytochemicals and antioxidant activities of conventionally propagated nodal segment and in vitro-induced callus of Bougainvillea glabra Choisy using different solvents. Horticulturae, 8 (8). art. no. 712. pp. 1-18. ISSN 2311-7524

Abstract

Bougainvillea, popularly known as ‘Bunga kertas’ in Malaysia, is thoroughly explored for nutritional and medicinal purposes. Bougainvillea has been shown to possess alkaloids and flavonoids which are widely used in folk medicine to treat different illnesses such as inflammatory, diarrheal, ulcer, and diabetic. Despite its major conventional therapeutic importance, only limited attempts have been made to investigate this species’ chemical and pharmacological properties in relation to its medicinal uses. Therefore, this study was conducted to determine the effect of in vitro-induced callus under different light conditions and plant growth regulators on phytochemical and antioxidant activities using different extraction solvents. Based on the results, the maximum days (17.67) to callus initiation were recorded when nodal was cultured on woody plant medium (WPM) supplemented with 7.5 µM 2,4-D + 0.5 µM BAP under light condition. On the contrary, the minimum days (7) to callus initiation were obtained when nodal was treated with 2.5 and 5 µM 2,4-D + 1 and 1.5 µM BAP under dark conditions. However, higher fresh and dry weight of callus was obtained when nodal was cultured on woody plant medium fortified with 7.5 µM 2,4-D + 1.5 µM BAP under dark and light conditions. In the analysis of the phenolics content and antioxidant activities, aqueous extract of conventionally propagated nodal part exhibited the highest phenolic content and antioxidant activities. However, the highest iron (II) chelating activity was produced from the aqueous extract of the calli induced under a dark condition. Hence, it can be concluded that the callus culture of Bougainvillea produced plant secondary metabolites and antioxidant activities comparable to the mother plants.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Institute of Tropical Forestry and Forest Products
DOI Number: https://doi.org/10.3390/horticulturae8080712
Publisher: MDPI
Keywords: Ornamental plant; Cytokinin; Auxin; Incubation condition; Callus induction; Antioxidant; Phenolic
Depositing User: Mr. Mohamad Syahrul Nizam Md Ishak
Date Deposited: 22 Jun 2024 14:11
Last Modified: 22 Jun 2024 14:11
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/horticulturae8080712
URI: http://psasir.upm.edu.my/id/eprint/102780
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