Citation
Mohd Salleh, Mohd Hairul and Esa, Yuzine
(2022)
Minimally invasive blood collection techniques as a source of gDNA for genetic studies on turtles and tortoises.
International Journal of Aquatic Biology, 10 (2).
145 - 150.
ISSN 2383-0956; ESSN: 2322-5270
Abstract
This technical note aims to describe the venipuncture procedure used to collect blood from Southern River terrapins via the subcarapacial venous plexus (SVP) and jugular vein. On uncooperative terrapins, SVP was applied while the jugular vein was reversed. 1.5 ml blood was preserved in 0.5 ml EDTA and stored at -20°C. ReliaPrepTM Blood genomic DNA Miniprep was used to extract DNA. Thermo ScientificTM NanoDrop 2000c was used to determine the concentrations of extracted DNAs. The greatest concentration of DNA is 136.3 g/L, and the highest purity is 1.90. The treatment is safe, minimally invasive, and effective.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Agriculture |
| Publisher: | Iranian society of Ichthyology |
| Keywords: | Venipuncture procedure; Southern River terrapin; Subcarapacial venous plexus; Jugular vein; Genomic |
| Depositing User: | Ms. Che Wa Zakaria |
| Date Deposited: | 10 Jul 2023 01:27 |
| Last Modified: | 10 Jul 2023 01:27 |
| URI: | http://psasir.upm.edu.my/id/eprint/102182 |
| Statistic Details: | View Download Statistic |
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