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Detection of porcine DNA in commercially processed meat products sold in Sarawak


Citation

Sajali, Nurhayatie and Mohd Desa, Mohd Nasir and Abu Bakar@Jamaludin, Suhaili and Wong, Sie Chuong and Bong, Ayyensia Sze Sze and Abg Koris, Dayangku Fariha Najiha and Liong, Kerry Sin Wei and Abdul Wahab, Abdul Fattah (2022) Detection of porcine DNA in commercially processed meat products sold in Sarawak. Halalsphere, 2 (2). pp. 8-16. ISSN 2773-6040

Abstract

Porcine DNA authentication in commercial products is critical due to improper or non-certified Halal logo on its packaging. This study detected the presence of porcine DNA in particularly processed meat products sold in Sarawak. A total of 75 samples were collected, consisting of poultry, beef, and seafood products. DNA was isolated and amplified in a polymerase chain reaction that targeted cytochrome B (cytb) and mitochondrial D-loop. PCR products were analysed via gel electrophoresis and viewed through gel documentation. The positive result was observed in 5 samples: 3 from poultry and 2 from beef. The DNA band were detected at an amplicon size of 174 base pairs for poultry products whereas 100 base pairs for beef products. Porcine DNA was absent in seafood products. Positive samples were validated through DNA sequencing. Nucleotide sequences from DNA sequencing were compared with the database using the Basic Local Alignment Search Tool (BLAST). BLAST analysis indicated percentages ranging from 96.83% to 100%, demonstrating high similarity with porcine DNA; one of the samples was labelled with a foreign Halal logo. In summary, this study provides the groundwork for further inspection of the Halal status in commercially processed meat products available in Sarawak.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Halal Products Research Institute
Faculty of Humanities, Management and Science
DOI Number: https://doi.org/10.31436/hs.v2i2.50
Publisher: IIUM Press
Keywords: Commercially processed meat products; Halal authentication; Mitochondrial genes; Polymerase chain reaction
Depositing User: Ms. Nur Faseha Mohd Kadim
Date Deposited: 20 Jul 2023 02:10
Last Modified: 20 Jul 2023 02:10
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.31436/hs.v2i2.50
URI: http://psasir.upm.edu.my/id/eprint/100920
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