Raja Abdul Rahman, Raja Noor Zaliha and Lee, Poh Geok and Basri, Mahiran and Salleh, Abu Bakar (2005) Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K. Bioresource Technology, 96 (4). pp. 429-436. ISSN 6097–6104
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Official URL: http://dx.doi.org/10.1016/j.biortech.2004.06.012
Abstract
The physical factors affecting the production of an organic solvent-tolerant protease from Pseudomonas aeruginosa strain K was investigated. Growth and protease production were detected from 37 to 45 °C with 37 °C being the optimum temperature for P. aeruginosa. Maximum enzyme activity was achieved at static conditions with 4.0% (v/v) inoculum. Shifting the culture from stationary to shaking condition decreased the protease production (6.0–10.0% v/v). Extracellular organic solvent-tolerant protease was detected over a broad pH range from 6.0 to 9.0. However, the highest yield of protease was observed at pH 7.0. Neutral media increased the protease production compared to acidic or alkaline media
| Item Type: | Article |
|---|---|
| Keyword: | Organic solvent-tolerant protease, Physical factors, Pseudomonas aeruginosa |
| Faculty or Institute: | Faculty of Environmental Studies |
| Publisher: | Elsevier |
| DOI Number: | 10.1016/j.biortech.2004.06.012 |
| ID Code: | 7075 |
| Deposited By: | Erni Suraya Abdul Aziz |
| Deposited On: | 07 Jun 2010 14:53 |
| Last Modified: | 27 Jul 2010 17:24 |
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