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Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K

Raja Abdul Rahman, Raja Noor Zaliha and Lee, Poh Geok and Basri, Mahiran and Salleh, Abu Bakar (2005) Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K. Bioresource Technology, 96 (4). pp. 429-436. ISSN 0960-8524; ESSN: 1873-2976

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Abstract

The physical factors affecting the production of an organic solvent-tolerant protease from Pseudomonas aeruginosa strain K was investigated. Growth and protease production were detected from 37 to 45 °C with 37 °C being the optimum temperature for P. aeruginosa. Maximum enzyme activity was achieved at static conditions with 4.0% (v/v) inoculum. Shifting the culture from stationary to shaking condition decreased the protease production (6.0–10.0% v/v). Extracellular organic solvent-tolerant protease was detected over a broad pH range from 6.0 to 9.0. However, the highest yield of protease was observed at pH 7.0. Neutral media increased the protease production compared to acidic or alkaline media.

Item Type:Article
Keyword:Organic solvent-tolerant protease; Physical factors; Pseudomonas aeruginosa
Faculty or Institute:Faculty of Science and Environmental Studies
Publisher:Elsevier
DOI Number:10.1016/j.biortech.2004.06.012
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.biortech.2004.06.012
ID Code:7075
Deposited By: Erni Suraya Abdul Aziz
Deposited On:07 Jun 2010 14:53
Last Modified:28 Sep 2016 15:03

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