Mat Radzi, Salina and Basri, Mahiran and Salleh, Abu Bakar and Ariff, Arbakariya and Mohammad, Rosfarizan and Abdul Rahman, Mohd Basyaruddin and Raja Abdul Rahman, Raja Noor Zaliha (2005) High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica. Electronic Journal of Biotechnology, 8 (3). ISSN 0717-3458
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Official URL: http://dx.doi.org/10.2225/vol8-issue3-fulltext-4
Abstract
High performance enzymatic synthesis of oleyl oleate, a liquid wax ester was carried out by lipase-catalysed esterification of oleic acid and oleyl alcohol. Various reaction parameters were optimised to obtain high yield of oleyl oleate. The optimum condition to produce oleyl oleate was reaction time; 5 min, organic solvents of log P ≥ 3.5, temperature; 40-50ºC, amount of enzyme; 0.2-0.4 g and molar ratio of oleyl alcohol to oleic acid; 2:1. The operational stability of enzyme was maintained at >90% yield up to 9 cycles. Analysis of the yield of the product showed that at optimum conditions, >95% liquid wax esters were produced.
| Item Type: | Article |
|---|---|
| Keyword: | Oleyl oleate, immobilised lipase, esterification, liquid wax ester |
| Faculty or Institute: | Faculty of Environmental Studies |
| Publisher: | Pontificia Universidad Católica de Valparaíso |
| DOI Number: | 10.2225/vol8-issue3-fulltext-4 |
| ID Code: | 7056 |
| Deposited By: | Erni Suraya Abdul Aziz |
| Deposited On: | 04 Jun 2010 14:43 |
| Last Modified: | 27 Jul 2010 17:20 |
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