High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica

Mat Radzi, Salina and Basri, Mahiran and Salleh, Abu Bakar and Ariff, Arbakariya and Mohammad, Rosfarizan and Abdul Rahman, Mohd Basyaruddin and Raja Abdul Rahman, Raja Noor Zaliha (2005) High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica. Electronic Journal of Biotechnology, 8 (3). ISSN 0717-3458

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Official URL: http://dx.doi.org/10.2225/vol8-issue3-fulltext-4

Abstract

High performance enzymatic synthesis of oleyl oleate, a liquid wax ester was carried out by lipase-catalysed esterification of oleic acid and oleyl alcohol. Various reaction parameters were optimised to obtain high yield of oleyl oleate. The optimum condition to produce oleyl oleate was reaction time; 5 min, organic solvents of log P ≥ 3.5, temperature; 40-50ºC, amount of enzyme; 0.2-0.4 g and molar ratio of oleyl alcohol to oleic acid; 2:1. The operational stability of enzyme was maintained at >90% yield up to 9 cycles. Analysis of the yield of the product showed that at optimum conditions, >95% liquid wax esters were produced.

Item Type:Article
Keyword:Oleyl oleate, immobilised lipase, esterification, liquid wax ester
Faculty or Institute:Faculty of Environmental Studies
Publisher:Pontificia Universidad Católica de Valparaíso
DOI Number:10.2225/vol8-issue3-fulltext-4
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.2225/vol8-issue3-fulltext-4
ID Code:7056
Deposited By: Erni Suraya Abdul Aziz
Deposited On:04 Jun 2010 06:43
Last Modified:27 Jul 2010 09:20

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