Bioassay-Guided Isolation and Identification of Bioactive Compounds from Garcinia Penangiana Leaves

Jabit, Mohd Lip (2005) Bioassay-Guided Isolation and Identification of Bioactive Compounds from Garcinia Penangiana Leaves. Masters thesis, Universiti Putra Malaysia.

[img] PDF
2637Kb

Abstract

Preliminary screening was done on 18 extracts of different parts of Garcinia sp. These extracts were tested on cytotoxic assay by using MTT Tetrazolium method on MCF-7 cells (hormone dependent breast cancer cells), DU145 (prostate cancer cells), H460 (non-small lung cancer) and HL60 (Leukemic cancer cells). Extracts of G. penangiana leaves, Garcinia urophylla leaves, G. maingayi leaves, G-maingayi stems and G. opaca fruits were found to have potent cytotoxic activity on MCF-7 cells and their ICso values are 5, 3, 6, 10 and 8 pg/mL, respectively. Furthermore, the extract of G. penangiana leaves also showed potent cytotoxic activity towards H460 cells (ICSo value of 8 clg/mL ). Bioassay guided isolation and purification led to the isolation of five xanthones and triterpene compounds fiom Garcinia penangiana leaves extracts. The triterpene and sterol isolated were charaterized as friedelin (23) and stigmasterol (24), respectively. The two isolated xanthones fiom the hexane hction were characterized as 1,3,5,8- tetrahydroxy-4-(1 ,I -dimethyl ally1)xanthone (25) and cudratricusxanthone H (26). The two xanthones isolated from the dichloromethane extract were characterised as 1,3,5,6- tetrahydroxy-2-(1,l -dimethylallyl)-4-(3-methyl-2-buteny1)xanthone or macluraxanthone C (27) and the new penangianaxanthone (25). Compound designated as 29 was also found as a mixture of 27 in dichloromethane fraction. The biosynthesis of 25,26 and 28 was suggested in the discussion. These compounds were tested for cytotoxic assay by using MTT Tetrazolium method on MCF-7 cells (hormone dependent breast cancer cells), DU145 (prostate cancer cells) and H460 (non-small lung cancer cells). Compound 25,26, 27,28 and mixture of 29 and 27 exhibited good and potent cytotoxic activity on MCF-7, NCI-H460 and DU145 cell lines. However, 23 and 24 showed no activity toward MCF-7 and NCI-H460 cell lines. 26,27, 28 and mixture of 29 and 27 showed similar pattern of cytotoxic activity toward MCF-7 cell line with ICso values of 3.9 f. 0.8, 3.1 + 0.1, 5.8 + 1.2 and 3 -0 f. 0.2 pg/mL, respectively. Similar patterns of cytotoxic activity were also observed when 25, 26, 27, 28 and mixture of 29 and 27 tested on NCI-H460 cell lines. The compounds showed ICso values of 13.4 + 1.1 pg/mL, 5.0 f. 1.2 pg/mL, 1.4 + 0.9pg/mL, 4.5 f 1.4 and 2.0 f 0.7 pg/mL, respectively. 26,27,28 and mixture of 29 and 27 showed similar pattern of cytotoxic activity toward DU145 cell line with their ICso values of 4.6 f 0.2,2.6 + 0.6, 4.3 + 0.4 MmL and 3.0 f 0.4 pg/mL, respectively.

Item Type:Thesis (Masters)
Subject:Garcinia
Subject:Biotechnology
Chairman Supervisor:Professor Nordin Hj Lajis, PhD
Call Number:IB 2005 12
Faculty or Institute:Institute of Bioscience
ID Code:6772
Deposited By: Nur Izyan Mohd Zaki
Deposited On:20 May 2010 07:29
Last Modified:27 May 2013 07:31

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 20 May 2010 07:29.

View statistics for "Bioassay-Guided Isolation and Identification of Bioactive Compounds from Garcinia Penangiana Leaves "


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.