Reliable and fast molecular sexing in Bos indicus cattle using amelogenin gene primers
Khaledi, Kourosh Jome and Panandam, Jothi Malar and Abdul Aziz, Maheran and Siraj, Siti Shapor (2009) Reliable and fast molecular sexing in Bos indicus cattle using amelogenin gene primers. Journal of Applied Animal Research, 35 (1). pp. 83-85. ISSN 0971-2119
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To investigate reliability of amelogenin gene primer pair for exon 5, SE47/SE48 for sex differentiation in Bos indicus three male and three female Brakmas (B. indicus) cattle were tested. The SE 47/48 primer pair generated PCR products of fragment length 280 bp for the females (AMELX) and fragments of 280 bp (AMELX) and 213 bp (AMELY) for the males. Thus the SE47/48 primer pair is not only reliable for sex differentiation in B. indicus but may also be used to differentiate the B. indicus and B. taurus males.
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