Simple Search:

Response surface methodology as a tool to study the lipase-catalyzed synthesis of betulinic acid ester


Citation

Yasin, Yamin and Basri, Mahiran and Ahmad, Faujan and Salleh, Abu Bakar (2008) Response surface methodology as a tool to study the lipase-catalyzed synthesis of betulinic acid ester. Journal of Chemical Technology and Biotechnology, 83 (5). pp. 694-698. ISSN 0268–2575

Abstract / Synopsis

BACKGROUND: The synthesis of betulinic acid ester using betulinic acid and oleyl alcohol catalyzed by Novozym 435 (immobilized Candida antarctica lipase) was carried out. Response surface methodology (RSM) based on a five-level, three-variable, central composite rotatable design (CCRD) was employed to evaluate the interactive effects of various parameters. The parameters were reaction time (8–16 h), temperature (20–60 ◦C) and enzyme amount (120–160 mg). RESULTS: Simultaneously increasing reaction time, temperature and amount of enzyme increased the yields of betulinic acid ester produced. CONCLUSION: The optimum conditions derived via RSM for the reaction were reaction time of 10.2 h, temperature of 53.1 ◦C and enzyme amount of 138 mg. The actual experimental yield was 48.5% under optimum conditions, which compared well with the maximum predicted value of 47.6%.


Download File

[img]
Preview
PDF (Abstract)
Response surface methodology as a tool to study the lipase.pdf

Download (150kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Science
DOI Number: 10.1002/jctb.1858
Publisher: Wiley Blackwell
Keywords: betulinic acid ester, enzymatic synthesis, lipase, response surface methodology (RSM)
Depositing User: Najwani Amir Sariffudin
Date Deposited: 13 May 2010 10:17
Last Modified: 11 Nov 2015 10:04
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1002/jctb.1858
URI: http://psasir.upm.edu.my/id/eprint/6325
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item