Response surface methodology as a tool to study the lipase-catalyzed synthesis of betulinic acid ester
Yasin, Yamin and Basri, Mahiran and Ahmad, Faujan and Salleh, Abu Bakar (2008) Response surface methodology as a tool to study the lipase-catalyzed synthesis of betulinic acid ester. Journal of Chemical Technology and Biotechnology, 83 (5). pp. 694-698. ISSN 0268–2575
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Official URL: http://dx.doi.org/10.1002/jctb.1858
BACKGROUND: The synthesis of betulinic acid ester using betulinic acid and oleyl alcohol catalyzed by Novozym 435 (immobilized Candida antarctica lipase) was carried out. Response surface methodology (RSM) based on a five-level, three-variable, central composite rotatable design (CCRD) was employed to evaluate the interactive effects of various parameters. The parameters were reaction time (8–16 h), temperature (20–60 ◦C) and enzyme amount (120–160 mg). RESULTS: Simultaneously increasing reaction time, temperature and amount of enzyme increased the yields of betulinic acid ester produced. CONCLUSION: The optimum conditions derived via RSM for the reaction were reaction time of 10.2 h, temperature of 53.1 ◦C and enzyme amount of 138 mg. The actual experimental yield was 48.5% under optimum conditions, which compared well with the maximum predicted value of 47.6%.
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