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Tissue engineering approach to repair abdominal wall defects using cell-seeded bovine tunica vaginalis in a rabbit model

Tsedeke, Ayele Taddese and Abu Bakar @ Zakaria, Md Zuki and Mohammed Alitheen, Noorjahan Banu and Mohamed Mustapha, Noordin (2010) Tissue engineering approach to repair abdominal wall defects using cell-seeded bovine tunica vaginalis in a rabbit model. Journal of Materials Science: Materials in Medicine, 21 (5). pp. 1721-1730. ISSN 0957-4530; ESSN: 1573-4838

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Official URL: http://dx.doi.org/10.1007/s10856-010-4007-7

Abstract

The aim of this study was to engineer skeletal muscle tissue for repair abdominal wall defects. Myoblast were seeded onto the scaffolds and cultivated in vitro for 5 days. Full thickness abdominal wall defects (3 × 4 cm) were created in 18 male New Zealand white rabbits and randomly divided into two equal groups. The defects of the first group were repaired with myoblast-seeded-bovine tunica vaginalis whereas the second group repaired with non-seeded-bovine tunica vaginalis and function as a control. Three animals were sacrificed at 7th, 14th, and 30th days of post-implantation from each group and the explanted specimens were subjected to macroscopic and microscopic analysis. In every case, seeded scaffolds have better deposition of newly formed collagen with neo-vascularisation than control group. Interestingly, multinucleated myotubes and myofibers were only detected in cell-seeded group. This study demonstrated that myoblast-seeded-bovine tunica vaginalis can be used as an effective scaffold to repair severe and large abdominal wall defects with regeneration of skeletal muscle tissue.

Item Type:Article
Keyword:Skeletal muscle tissue; Abdominal wall defect; Myoblast-seeded-bovine tunica vaginalis
Faculty or Institute:Faculty of Veterinary Medicine
Faculty of Biotechnology and Biomolecular Sciences
Publisher:Springer
DOI Number:10.1007/s10856-010-4007-7
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s10856-010-4007-7
ID Code:6175
Deposited By: Azizan Arshad
Deposited On:12 May 2010 16:02
Last Modified:16 Nov 2016 15:32

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